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Literature summary for 3.4.21.6 extracted from

  • Ludeman, J.P.; Pike, R.N.; Bromfield, K.M.; Duggan, P.J.; Cianci, J.; Le Bonniec, B.; Whisstock, J.C.; Bottomley, S.P.
    Determination of the P'1, P'2 and P'3 subsite-specificity of factor Xa (2003), Int. J. Biochem. Cell Biol., 35, 221-225.
    View publication on PubMed
Search for more literature for 3.4.21.6

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin scutelarin has similar specificity, most selective P'2-position, less selective P'1-position, and non-selective P'3-position Bos taurus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity with diverse fluorescence-quenched substrates Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Ile-Glu(gamma-OR)-Gly-Arg-4-nitroanilide + H2O
-
 Bos taurus benzoyl-Ile-Glu(gamma-OR)-Gly-Arg + 4-nitroaniline
-
 ?
GPRFFL + H2O best substrate Bos taurus GPR + FFL
-
 ?
additional information substrate specificity study for the positions P'1, P'2, and P'3 with 57 substrates possesssing Gly-Pro-Arg at positions P1-P3, the P'2 position shows the highest selectivity, while the P'3 position is not selective at all, overview Bos taurus ?
-
 ?
prothrombin + H2O
-
 Bos taurus thrombin + activation peptide
-
 ?

Synonyms

Synonyms Comment Organism
factor X zymogen Bos taurus
factor Xa mature enzyme Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Bos taurus