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Literature summary for 3.4.21.6 extracted from

  • O'Brien, L.A.; Stafford, A.R.; Fredenburgh, J.C.; Weitz, J.I.
    Glycosaminoglycans bind factor Xa in a Ca2+-dependent fashion and modulate its catalytic activity (2003), Biochemistry, 42, 13091-13098.
    View publication on PubMed
Search for more literature for 3.4.21.6

Activating Compound

Activating Compound Comment Organism Structure
Glycosaminoglycans bind with higher affinity to factor Xa in presence of Ca2+ than in its absence, while the binding to the Gla-domainless factor Xa shows similar Km-values in absence or presence of Ca2+, binding induces conformational changes in the active site of factor Xa, glycosaminoglycans influence the ability of factor Xa to cleave chromogenic substrates and reduce its factor VII cleaving activity, exosites on the surface of factor Xa are perturbed, the activation effect increass with increasing number of sulfate residues Homo sapiens
heparin binds to a Ca2+-dependent binding site, reduces Km and kcat for chromogenic substrates dependent on Ca2+ Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information binding kinetics of heparin and other glycosaminoglycans to the active site-blocked enzyme Homo sapiens
0.042
-
 N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide pH 7.4, 23°C, in presence of 0.001 mM heparin Homo sapiens
0.057
-
 N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide pH 7.4, 23°C, in absence of heparin Homo sapiens
0.097
-
 tosyl-glycyl-prolyl-D-arginine-4-nitroanilide pH 7.4, 23°C, in presence of 0.001 mM heparin Homo sapiens
0.206
-
 tosyl-glycyl-prolyl-D-arginine-4-nitroanilide pH 7.4, 23°C, in absence of heparin Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin scutelarin has similar specificity Homo sapiens
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide + H2O i.e. S-2765, synthetic chromogenic substrate Homo sapiens N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine + 4-nitroaniline
-
 ?
tosyl-glycyl-prolyl-D-arginine-4-nitroanilide + H2O i.e. Chz-TH, synthetic chromogenic substrate Homo sapiens tosyl-glycyl-prolyl-D-arginine + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
f.Xa
-
 Homo sapiens
factor Xa
-
 Homo sapiens
More the enzyme belongs to the peptidase family S1, i.e. trypsin family Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
46
-
 tosyl-glycyl-prolyl-D-arginine-4-nitroanilide pH 7.4, 23°C, in presence of 0.001 mM heparin Homo sapiens
79
-
 tosyl-glycyl-prolyl-D-arginine-4-nitroanilide pH 7.4, 23°C, in absence of heparin Homo sapiens
144
-
 N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide pH 7.4, 23°C, in presence of 0.001 mM heparin Homo sapiens
169
-
 N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide pH 7.4, 23°C, in absence of heparin Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens