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Literature summary for 3.4.21.59 extracted from

  • Dacre, K.J.; McAleese, S.M.; Knight, P.; McGorum, B.C.; Pemberton, A.D.
    cDNA cloning and substrate specificity of equine tryptase, a possible mediator in equine heaves (2006), Clin. Exp. Allergy, 36, 1303-1309.
    View publication on PubMed
Search for more literature for 3.4.21.59

Cloned(Commentary)

Cloned (Comment) Organism
gene mtc1, DNA and amino acid sequence determination and analysis, equine tryptase has alanine at residue 216, rather than glycine, which confers increased arginine substrate specificity in vitro and may restrict fibrinogenolysis in vivo Equus caballus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Equus caballus
0.922
-
 N-(p-tosyl)-Gly-Pro-Arg-4-nitroanilide pH 8.0, 22°C Equus caballus
2.849
-
 N-(p-tosyl)-Gly-Pro-Lys-p-nitroanilide pH 8.0, 22°C Equus caballus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fibrinogen + H2O Equus caballus
-
 fibrin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Equus caballus Q7YS62 gene mtc1; gene mtc1
-

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
 Equus caballus
-
mast cell
-
 Equus caballus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fibrinogen + H2O
-
 Equus caballus fibrin + ?
-
 ?
additional information equine tryptase has alanine at residue 216, rather than glycine, which confers increased arginine substrate specificity in vitro and may restrict fibrinogenolysis in vivo Equus caballus ?
-
 ?
N-(p-tosyl)-Gly-Pro-Arg-4-nitroanilide + H2O
-
 Equus caballus N-(p-tosyl)-Gly-Pro-Arg + 4-nitroaniline
-
 ?
N-(p-tosyl)-Gly-Pro-Lys-4-nitroanilide + H2O
-
 Equus caballus N-(p-tosyl)-Gly-Pro-Lys + 4-nitroaniline
-
 ?
S2366 + H2O
-
 Equus caballus ?
-
 ?

Synonyms

Synonyms Comment Organism
Mast cell tryptase
-
 Equus caballus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Equus caballus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12
-
 N-(p-tosyl)-Gly-Pro-Lys-p-nitroanilide pH 8.0, 22°C Equus caballus
23.5
-
 N-(p-tosyl)-Gly-Pro-Arg-4-nitroanilide pH 8.0, 22°C Equus caballus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 assay at Equus caballus