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Literature summary for 3.4.21.59 extracted from

  • Schechter, N.M.; Choi, E.J.; Selwood, T.; McCaslin, D.R.
    Characterization of three distinct catalytic forms of human tryptase-beta: their interrelationships and relevance (2007), Biochemistry, 46, 9615-9629.
    View publication on PubMed
Search for more literature for 3.4.21.59

Activating Compound

Activating Compound Comment Organism Structure
heparin is required for stabilization of the enzyme tetramer as a prerequisite for catalytic activity, the enzyme activity is 70fold increased, pH-dependent complex formation and complex structure, overview Homo sapiens

General Stability

General Stability Organism
heparin stabilizes the active tetramer Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
BPTI irreversible complex formation between protease and serpin Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics and binding constants Homo sapiens
0.062
-
 D-Ile-Pro-Arg-4-nitroanilide pH 8.0, 25°C, active enzyme tetramer, in presence of heparin and Na+ Homo sapiens
0.081
-
 D-Ile-Pro-Arg-4-nitroanilide pH 6.8, 25°C, active enzyme tetramer, in presence of heparin and Na+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 three distinct catalytic forms of human tryptase-beta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha2-antiplasmin + H2O substrate binding and interaction with the enzyme, overview Homo sapiens ?
-
 ?
D-Ile-Pro-Arg-4-nitroanilide + H2O
-
 Homo sapiens D-Ile-Pro-Arg + 4-nitroaniline
-
 ?
Fibrinogen + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More activity of different enzyme conformations, overview Homo sapiens
tetramer a homotetramer of four catalytically active subunits, unless stabilized by heparin or high salt, the active tetramer converts to an inactive state consisting of an inactive-destabilized tetramer that reversibly dissociates to inactive monomers upon dilution, overview Homo sapiens

Synonyms

Synonyms Comment Organism
HTbeta
-
 Homo sapiens
tryptase-beta
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
97
-
 D-Ile-Pro-Arg-4-nitroanilide pH 6.8, 25°C, active enzyme tetramer, in presence of heparin and Na+ Homo sapiens
170
-
 D-Ile-Pro-Arg-4-nitroanilide pH 8.0, 25°C, active enzyme tetramer, in presence of heparin and Na+ Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8.5 in complex with heparin Homo sapiens