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Literature summary for 3.4.21.59 extracted from

  • Chan, H.; Elrod, K.C.; Numerof, R.P.; Sideris, S.; Clark, J.M.
    Expression and characterization of recombinant mast cell tryptase (1999), Protein Expr. Purif., 15, 251-257.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cDNA cloned and expressed in the methylotrophic yeast Pichia pastoris as protryptase Homo sapiens

General Stability

General Stability Organism
stabilized by heparin proteoglycans Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
APC-1167
-
Homo sapiens
APC-366
-
Homo sapiens
Aprotinin weak inhibitor Homo sapiens
BABIM
-
Homo sapiens
additional information no inhibition of native or recombinant enzyme by soybean trypsin inhibitor SBTI Homo sapiens
Pentamidine nonselective inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5
-
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 8.2, 22°C, recombinant tryptase Homo sapiens
4.2
-
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 8.2, 22°C, HMC-1 tryptase Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
secretory granule
-
Homo sapiens 30141
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
human
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fibrinogen + H2O
-
Homo sapiens fibrinogen fragments
-
?
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O synthetic peptide substrate Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
tetramer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
HMC-1 tryptase
-
Homo sapiens
Mast cell tryptase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
490
-
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 8.2, 22°C, recombinant tryptase Homo sapiens
540
-
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 8.2, 22°C, HMC-1 tryptase Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0017
-
APC-366 pH 8.2, 22°C, recombinant tryptase Homo sapiens
0.0023
-
Pentamidine pH 8.2, 22°C, recombinant tryptase Homo sapiens
0.0025
-
BABIM pH 8.2, 22°C, recombinant tryptase Homo sapiens
0.0028
-
Pentamidine pH 8.2, 22°C Homo sapiens
0.0028
-
APC-366 pH 8.2, 22°C Homo sapiens
0.0046
-
BABIM pH 8.2, 22°C Homo sapiens
0.0092
-
APC-1167 pH 8.2, 22°C, recombinant tryptase Homo sapiens
0.0097
-
APC-1167 pH 8.2, 22°C Homo sapiens
0.25
-
Aprotinin pH 8.2, 22°C Homo sapiens
0.369
-
Aprotinin pH 8.2, 22°C, recombinant tryptase Homo sapiens