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Literature summary for 3.4.21.59 extracted from

  • Selwood, T.; Wang, Z.M.; McCaslin, D.R.; Schechter, N.M.
    Diverse stability and catalytic properties of human tryptase alpha and beta isoforms are mediated by residue differences at the S1 pocket (2002), Biochemistry, 41, 3329-3340.
    View publication on PubMed
Search for more literature for 3.4.21.59

Protein Variants

Protein Variants Comment Organism
D216G active site variant of rHTalpha Homo sapiens
E149T active site variant of rHTbeta Homo sapiens
K192Q active site variant of rHTalpha Homo sapiens
K192Q/D216G active site variant of rHTalpha Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 4 * 30000, rHTalpha, mutant D216G, SDS-PAGE, recombinant rHTbeta Homo sapiens
150000
-
 rHT, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-

Purification (Commentary)

Purification (Comment) Organism
HST and rHT Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity for hydrolysis of model substrates by HAST and rHTs, micromol of nitroanilide min-1/nmol subunit Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbobenzoxy-GPR-4-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
Nalpha-benzoyl-L-arginine-p-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
tosyl-GPK-4-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
tosyl-GPR-4-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 30000, rHTalpha, mutant D216G, SDS-PAGE, recombinant rHTbeta Homo sapiens

Synonyms

Synonyms Comment Organism
HAST
-
 Homo sapiens
human tryptase alpha
-
 Homo sapiens
human tryptase beta
-
 Homo sapiens
rHT
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 HST, half-life 24 min, rHtbeta 8.5 min, rHTbeta-E149T 7.5 min, rHTalpha-K192Q-D216G 35 min Homo sapiens
30
-
 HST, half-life 4.3 min, rHtbeta 1.7 min, rHTbeta-E149T 2.3 min, rHTalpha-D216G 385 min, rHTalpha-K192Q-D216G 14 min Homo sapiens
37
-
 HST, half-life 1 min, rHtbeta 0.5 min, rHTbeta-E149T 1.0 min, rHTalpha-D216G 85 min, rHTalpha-K192Q-D216G 1.8 min Homo sapiens