Literature summary for 3.4.21.59 extracted from

Alter, S.C.; Metcalfe, D.D.; Bradford, T.R.; Schwartz, L.B.
Regulation of human mast cell tryptase. Effects of enzyme concentration, ionic strength and the structure and negative charge density of polysaccharides (1987), Biochem. J., 248, 821-827.

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General Stability

General Stability	Organism
chondroitin monosulfate stabilizes, prolongs t1/2 at 37°C 20fold	Homo sapiens
chondroitin sulfate E stabilizes, prolongs t1/2 at 37°C 69fold	Homo sapiens
heparan sufate stabilizes, prolongs t1/2 at 37°C 20fold	Homo sapiens
heparin glycosaminoglycan fragments of MW greater than 5700 stabilizes	Homo sapiens
increasing the NaCl concentration from 0.01 M to 1.0 M increases stability of free tryptase, in the presence of stabilizing polysaccharides it decreases the stability of tryptase until dissociation of tryptase from each polysaccharide occurs, thereafter tryptase stability increases as does that of free tryptase	Homo sapiens
poly-D-glutamic acid stabilizes, prolongs t1/2 at 37°C 55fold: Dextran sulfate and heparin provide complete stabilization of tryptase activity for 120 min at 37°C	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	effect of NaCl on Km	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Tosyl-Arg methyl ester + H2O	-	Homo sapiens	?	-	?
Tosyl-Gly-Pro-Lys 4-nitroanilide + H2O	-	Homo sapiens	Tosyl-Gly-Pro-Lys + 4-nitroaniline	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	effect of NaCl on turnover number	Homo sapiens

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Release 2023.1 (January 2023)