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Literature summary for 3.4.21.55 extracted from

  • Laraba-Diebari, F.; Martin-Eauclaire, M.F.; Mauco, G.; Marchot, P.
    Afaacytin, an alpha beta-fibrinogenase from Cerastes cerastes (horned viper) venom, activates purified factor X and induces serotonin release from human blood platelets (1995), Eur. J. Biochem., 233, 756-765.
    View publication on PubMed
Search for more literature for 3.4.21.55

Inhibitors

Inhibitors Comment Organism Structure
benzamidine
-
 Cerastes cerastes
diisopropyl fluorophosphate inhibits esterase activity Cerastes cerastes
EGTA Ca2+ does not restore Cerastes cerastes
additional information heparin or hirudin; no inhibition by plasmic thrombin inhibitors, antithrombin III, ecotin Cerastes cerastes

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Cerastes cerastes
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for structural cohesion of afaâcytin molecule Cerastes cerastes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Cerastes cerastes
10200
-
 1 * 40500 + 1 * 35500 + 1 * 10200, Cerastes cerastes, alphabetabeta, SDS-PAGE, reducing conditions, 1 * 38250 + 1 * 28500 + 1 * 10200, Cerastes cerastes, deglycosylated enzyme, alphabetabeta, SDS-PAGE, reducing conditions Cerastes cerastes
28500
-
 1 * 40500 + 1 * 35500 + 1 * 10200, Cerastes cerastes, alphabetabeta, SDS-PAGE, reducing conditions, 1 * 38250 + 1 * 28500 + 1 * 10200, Cerastes cerastes, deglycosylated enzyme, alphabetabeta, SDS-PAGE, reducing conditions Cerastes cerastes
35500
-
 1 * 40500 + 1 * 35500 + 1 * 10200, Cerastes cerastes, alphabetabeta, SDS-PAGE, reducing conditions, 1 * 38250 + 1 * 28500 + 1 * 10200, Cerastes cerastes, deglycosylated enzyme, alphabetabeta, SDS-PAGE, reducing conditions Cerastes cerastes
38250
-
 1 * 40500 + 1 * 35500 + 1 * 10200, Cerastes cerastes, alphabetabeta, SDS-PAGE, reducing conditions, 1 * 38250 + 1 * 28500 + 1 * 10200, Cerastes cerastes, deglycosylated enzyme, alphabetabeta, SDS-PAGE, reducing conditions Cerastes cerastes
40000
-
 2 * 40000, Cerastes cerastes, alphabeta (where beta consists of two disufide linked polypeptide chains beta and beta), SDS-PAGE Cerastes cerastes
40500
-
 1 * 40500 + 1 * 35500 + 1 * 10200, Cerastes cerastes, alphabetabeta, SDS-PAGE, reducing conditions, 1 * 38250 + 1 * 28500 + 1 * 10200, Cerastes cerastes, deglycosylated enzyme, alphabetabeta, SDS-PAGE, reducing conditions Cerastes cerastes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fibrinogen + H2O Cerastes cerastes promotes human platelet aggregation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cerastes cerastes
-
 Horned viper
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein alpha and beta subunits are N-glycosylated, not beta' Cerastes cerastes

Purification (Commentary)

Purification (Comment) Organism
-
 Cerastes cerastes

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Cerastes cerastes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Cerastes cerastes hydrolyzed casein
-
 ?
Fibrin alpha-chain + H2O
-
 Cerastes cerastes Hydrolyzed fibrin alpha-chain fragments of low molecular mass ?
Fibrinogen + H2O cleaves Aalpha- and Bbeta-chain, preferably Aalpha, no cleavage of gamma-chain Cerastes cerastes Fibrinopeptide A + fibrinopeptide B + ?
-
 ?
Fibrinogen + H2O promotes human platelet aggregation Cerastes cerastes ?
-
 ?
H-D-Cyclohexylglycyl-L-2-aminobutyryl-L-arginine 4-nitroanilide + H2O i.e. CBS 34-47, chromogenic substrate Cerastes cerastes H-D-Cyclohexylglycyl-L-alpha-aminobutyryl-L-arginine + 4-nitroaniline
-
 ?
Human blood coagulation factor X + H2O enzyme appears to act as substitute for both factor VIIIa and IXa by activating factor X Cerastes cerastes Human blood coagulation factor Xa i.e. EC 3.4.21.6 ?
additional information strong arginine esterase and amidase activity on synthetic substrates Cerastes cerastes ?
-
 ?
additional information the enzyme from Cerastes cerastes exhibits alphabeta-fibrinogenase and alpha-fibrinase properties Cerastes cerastes ?
-
 ?
additional information no hydrolysis of fibrin gamma-chains, fibrin beta-chains or gammagamma dimer Cerastes cerastes ?
-
 ?
additional information releases serotonin from previously loaded platelets Cerastes cerastes ?
-
 ?
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
-
 Cerastes cerastes ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40000, Cerastes cerastes, alphabeta (where beta consists of two disufide linked polypeptide chains beta and beta'), SDS-PAGE Cerastes cerastes
trimer 1 * 40500 + 1 * 35500 + 1 * 10200, Cerastes cerastes, alphabetabeta', SDS-PAGE, reducing conditions, 1 * 38250 + 1 * 28500 + 1 * 10200, Cerastes cerastes, deglycosylated enzyme, alphabetabeta', SDS-PAGE, reducing conditions Cerastes cerastes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
 assay at Cerastes cerastes
27
-
 factor X as substrate Cerastes cerastes
37
-
 fibrinogen as substrate Cerastes cerastes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 6.25 Cerastes cerastes