Cloned (Comment) | Organism |
---|---|
gene lon, functional recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain CH1019 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
S679A | site-directed mutagenesis, the Lon trapping variant is able to translocate substrates but unable to degrade them, it is established and used for substrate determinations by mass spectrometry | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CysB + H2O | Escherichia coli | a positive cysDNC operon transcription regulator | ? | - |
? | |
CysD + H2O | Escherichia coli | a subunit of the sulfate adenylyltransferase, low activity | ? | - |
? | |
GlyA + H2O | Escherichia coli | a protein of the MetR regulon | ? | - |
? | |
MetR + H2O | Escherichia coli | a protein of the MetR regulon, transcriptional regulator of metE expression | ? | - |
? | |
additional information | Escherichia coli | a quantitative Super-SILAC (stable isotope labeling with amino acids in cell culture) mass spectrometry approach and analysis of proteomes of a lon mutant and a strain producing the protease are employed to determine substrate specificity and Lon-dependent physiological functions. The recognition mechanisms of known Lon substrates are highly diverse. Misfolded proteins are mainly recognized by short, hydrophobic stretches normally buried in the core of natively folded proteins. In contrast, recognition of SulA occurs via its C-terminus with a critical histidine and tyrosine at its very end | ? | - |
? | |
SulA + H2O | Escherichia coli | a cell division inhibitor | ? | - |
? | |
ZntR + H2O | Escherichia coli | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A9M0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain CH1019 by nickel affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CysB + H2O | a positive cysDNC operon transcription regulator | Escherichia coli | ? | - |
? | |
CysD + H2O | a subunit of the sulfate adenylyltransferase, low activity | Escherichia coli | ? | - |
? | |
GlyA + H2O | a protein of the MetR regulon | Escherichia coli | ? | - |
? | |
MetR + H2O | a protein of the MetR regulon | Escherichia coli | ? | - |
? | |
MetR + H2O | a protein of the MetR regulon, transcriptional regulator of metE expression | Escherichia coli | ? | - |
? | |
additional information | a quantitative Super-SILAC (stable isotope labeling with amino acids in cell culture) mass spectrometry approach and analysis of proteomes of a lon mutant and a strain producing the protease are employed to determine substrate specificity and Lon-dependent physiological functions. The recognition mechanisms of known Lon substrates are highly diverse. Misfolded proteins are mainly recognized by short, hydrophobic stretches normally buried in the core of natively folded proteins. In contrast, recognition of SulA occurs via its C-terminus with a critical histidine and tyrosine at its very end | Escherichia coli | ? | - |
? | |
SulA + H2O | - |
Escherichia coli | ? | - |
? | |
SulA + H2O | a cell division inhibitor | Escherichia coli | ? | - |
? | |
ZntR + H2O | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent lon protease | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | a Lon trapping variant, which is able to translocate substrates but unable to degrade them, is established and used for substrate determinations by mass spectrometry | Escherichia coli |
physiological function | a quantitative Super-SILAC (stable isotope labeling with amino acids in cell culture) mass spectrometry approach and analysis of proteomes of a lon mutant and a strain producing the protease are employed to determine substrate specificity and Lon-dependent physiological functions, Lon affected proteins, overview. Fundamental functions of Lon in sulfur assimilation, nucleotide biosynthesis, amino acid and central energy metabolism, besides the superoxide stress response function. Lon protease affects the MetR regulon and function of proteins MetE and MetR | Escherichia coli |