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Literature summary for 3.4.21.53 extracted from
- Three-dimensional reconstruction of the S885A mutant of human mitochondrial Lon protease (2014), Folia Biol. (Praha), 60 Suppl 1, 62-65.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His-tagged wild-type and mutant enzyme in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S885A | site-directed mutagenesis, three-dimensional structure of the ADP-bound Lon S885A mutant obtained by electron microscopy as a result of preliminary negative staining studies | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stabilizes the enzyme complex | Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by ultracentrifugation, nickel affinity chromatography and gel filtration | Homo sapiens |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer or heptamer | stabilized by Mg2+ and ADP bound to the ATPase region | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lon | - |
Homo sapiens |
| lon protease | - |
Homo sapiens |
| Lon protein | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | ADP bound to the ATPase region stabilizes the enzyme | Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme is a member of the ATPase superfamily | Homo sapiens |
| malfunction | altered expression levels of the enzyme are linked to some severe diseases such as epilepsy, myopathy, or lateral sclerosis | Homo sapiens |
| physiological function | the main function of the enzyme is the control of protein quality and the maintenance of proteostasis by degradation of misfolded and damaged proteins, which occur in response to numerous stress conditions. It also participates in the regulation of levels of transcription factors that control pathogenesis, development and stress response | Homo sapiens |
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Release 2023.1 (January 2023)
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