Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | oligomerization is stimulated by unfolded protein | Mycolicibacterium smegmatis | |
additional information | protein substrate stimulates DNA binding | Homo sapiens | |
Polyphosphate | stimulates lon-mediated proteolysis of free ribosomal proteins and thereby down-regulates translation | Escherichia coli |
Application | Comment | Organism |
---|---|---|
additional information | functions in protein quality control | Escherichia coli |
additional information | induction of lon contributes to the adaptive resistance of the organism towards antibiotic treatment | Pseudomonas aeruginosa |
additional information | stress response network between the endoplasmic reticulum, nucleus and mitochondrion up-regulates the expression, thereby providing a cytoprotective function during endoplasmic reticulum stress and hypoxia | Homo sapiens |
additional information | stress response network between the endoplasmic reticulum, nucleus and mitochondrion up-regulates the expression, thereby providing a cytoprotective function during endoplasmic reticulum stress and hypoxia | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E614K | mixed oligomeric complexes composed of wild-type lon and the inactive lon E614K mutant, results in an enzymatically inactive protein | Escherichia coli |
additional information | absence of lon, results in a lack of ATP-dependent proteolysis in the mitochondrial matrix, accumulation of electron dense aggregates and large mitochondrial DNA deletions. Mutant lacking both ATPase and protease activity also fails to suppress COX assembly defects | Saccharomyces cerevisiae |
additional information | lon gene mutants, form long undivided filaments upon UV irradiation | Escherichia coli |
additional information | lon-depleted cells show little if any mitochondrial DNA damage | Homo sapiens |
S679W | proteolytically inactive mutant. ATPase activity is affected by a variety of mutations generated at the vicinity of the proteolytic site Ser 679. Mutation of the ATP-binding site abolishes both the ATPase and protease activities of lon | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
anti-sense morpholino oligonucleotide | causes defects in mitochondrial membrane potential, respiration and morphology, as well as apoptotic cell death | Homo sapiens | |
ATP | ATP-binding inhibits DNA binding | Homo sapiens | |
bacteriophage T4 PinA protein | specifically inhibits Escherichia coli Lon | Escherichia coli | |
MG262 | i.e. Cb2-Leu-Leu-Leu-bornic acid | Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | expression of an inducible short hairpin RNA leading to lon depletion in a colon adenocarcinoma cell line for 14 days does not lead to cell death. Even after RNAi knockdown for 3 weeks, these cells continue to survive, although they no longer proliferate | Homo sapiens | |
Polyphosphate | competitively blocks DNA binding by lon in vitro and in vivo | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Escherichia coli | 5829 | - |
mitochondrion | - |
Homo sapiens | 5739 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | oligomerization is dependent on Mg2+ | Escherichia coli | |
Mg2+ | oligomerization is dependent on Mg2+ | Mycolicibacterium smegmatis | |
additional information | maintenance of the holoenzyme does not require the addition of Mg2+ | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brucella abortus | - |
- |
- |
Caulobacter vibrioides | - |
- |
- |
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Mycolicibacterium smegmatis | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Thermoplasma acidophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Saccharomyces cerevisiae |
- |
Mycolicibacterium smegmatis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colonic adenocarcinoma cell line | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bacteriophage lambda N protein + H2O | - |
Escherichia coli | ? | - |
? | |
casein + H2O | - |
Escherichia coli | ? | - |
? | |
CcdA + H2O | - |
Escherichia coli | ? | - |
? | |
Cox4-1 + H2O | - |
Homo sapiens | ? | - |
? | |
DNA methyltransferase + H2O | selectively degrades cell-cycle-regulated DNA methyltransferase thereby regulating methylation of chromosomal DNA and cellular differentiation | Caulobacter vibrioides | ? | - |
? | |
HilA + H2O | mediates proteolysis of the central transcription regulatory factor HilA, which controls the correct timing for the expression of virulence genes necessary for host invasion | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
HilA + H2O | mediates proteolysis of the central transcription regulatory factor HilA, which controls the correct timing for the expression of virulence genes necessary for host invasion | Brucella abortus | ? | - |
? | |
mitochondrial processing peptidase alpha subunit + H2O | is degraded only when it is folded, trypsin-resistant and competent for assembly into an active enzyme | Homo sapiens | ? | - |
? | |
additional information | endogenous substrates, which are misfolded or unassembled subunits of electron transport chain complexes, ribosomal proteins and metabolic enzymes | Saccharomyces cerevisiae | ? | - |
? | |
Rcs + H2O | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heptamer | cryoelectron microscopy and analytic ultracentrifugation | Saccharomyces cerevisiae |
hexamer | analytical ultracentrifugation | Mycolicibacterium smegmatis |
hexamer | electron microscopy | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent lon protease | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
ATP-dependent lon protease | - |
Escherichia coli |
ATP-dependent lon protease | - |
Homo sapiens |
ATP-dependent lon protease | - |
Rattus norvegicus |
ATP-dependent lon protease | - |
Saccharomyces cerevisiae |
ATP-dependent lon protease | - |
Mycolicibacterium smegmatis |
ATP-dependent lon protease | - |
Pseudomonas aeruginosa |
ATP-dependent lon protease | - |
Thermoplasma acidophilum |
ATP-dependent lon protease | - |
Brucella abortus |
ATP-dependent lon protease | - |
Caulobacter vibrioides |
lon | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
lon | - |
Escherichia coli |
lon | - |
Homo sapiens |
lon | - |
Rattus norvegicus |
lon | - |
Saccharomyces cerevisiae |
lon | - |
Mycolicibacterium smegmatis |
lon | - |
Pseudomonas aeruginosa |
lon | - |
Thermoplasma acidophilum |
lon | - |
Brucella abortus |
lon | - |
Caulobacter vibrioides |
lon protease | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
lon protease | - |
Escherichia coli |
lon protease | - |
Homo sapiens |
lon protease | - |
Rattus norvegicus |
lon protease | - |
Saccharomyces cerevisiae |
lon protease | - |
Mycolicibacterium smegmatis |
lon protease | - |
Pseudomonas aeruginosa |
lon protease | - |
Thermoplasma acidophilum |
lon protease | - |
Brucella abortus |
lon protease | - |
Caulobacter vibrioides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | maintenance of the holoenzyme does not require the addition of ATP. ATP binding induces conformational changes in the holoenzyme | Saccharomyces cerevisiae | |
additional information | oligomerization is independent of ATP | Escherichia coli | |
additional information | oligomerization is independent of ATP | Mycolicibacterium smegmatis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000066 | - |
MG262 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |