Activating Compound | Comment | Organism | Structure |
---|---|---|---|
adenylyl 5-imidodiphosphate | supports peptide hydrolysis by lon | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
mutants via plasmids pJW028 and pJW015 expressed in Escherichia coli BL21 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
S679A | proteolytically inactive, but wild-type-like intrinsic and peptide-stimulated ATPase activitiy. Two-step peptide S4 binding event, where a conformational change occurs after a rapid equilibrium peptide binding step | Escherichia coli |
S679W | proteolytically inactive, but wild-type-like intrinsic and peptide-stimulated ATPase activitiy. Two-step peptide S4 binding event, where a conformational change occurs after a rapid equilibrium peptide binding step | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
ATP | wild-type | Escherichia coli | |
0.079 | - |
ATP | mutant S679W with S3 peptide | Escherichia coli | |
0.086 | - |
ATP | wild-type with S2 peptide | Escherichia coli | |
0.131 | - |
ATP | mutant S679A with S3 peptide | Escherichia coli | |
0.139 | - |
ATP | mutant S679W | Escherichia coli | |
0.147 | - |
ATP | mutant S679A | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutants purified to homogeneity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Escherichia coli | phosphate + ADP | - |
? | |
YRGITCSGRQK(benzoic acid) + H2O | S2 peptide | Escherichia coli | ? | - |
? | |
YRGITCSGRQK-(dansyl) + H2O | S4 peptide | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lon | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
ATP | wild-type | Escherichia coli | |
0.31 | - |
ATP | mutant S679A | Escherichia coli | |
0.64 | - |
ATP | mutant S679W | Escherichia coli | |
1.07 | - |
ATP | mutant S679W with S3 peptide | Escherichia coli | |
1.43 | - |
ATP | wild-type with S2 peptide | Escherichia coli | |
1.57 | - |
ATP | mutant S679A with S3 peptide | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | optimal peptide cleavage by lon requires hydrolysis of ATP to fully activate the proteolytic site | Escherichia coli |