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Literature summary for 3.4.21.53 extracted from

  • Patterson-Ward, J.; Huang, J.; Lee, I.
    Detection and characterization of two ATP-dependent conformational changes in proteolytically inactive Escherichia coli Lon mutants by stopped flow kinetic techniques (2007), Biochemistry, 46, 13593-13605.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
adenylyl 5-imidodiphosphate supports peptide hydrolysis by lon Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
mutants via plasmids pJW028 and pJW015 expressed in Escherichia coli BL21 Escherichia coli

Protein Variants

Protein Variants Comment Organism
S679A proteolytically inactive, but wild-type-like intrinsic and peptide-stimulated ATPase activitiy. Two-step peptide S4 binding event, where a conformational change occurs after a rapid equilibrium peptide binding step Escherichia coli
S679W proteolytically inactive, but wild-type-like intrinsic and peptide-stimulated ATPase activitiy. Two-step peptide S4 binding event, where a conformational change occurs after a rapid equilibrium peptide binding step Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
ATP wild-type Escherichia coli
0.079
-
ATP mutant S679W with S3 peptide Escherichia coli
0.086
-
ATP wild-type with S2 peptide Escherichia coli
0.131
-
ATP mutant S679A with S3 peptide Escherichia coli
0.139
-
ATP mutant S679W Escherichia coli
0.147
-
ATP mutant S679A Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutants purified to homogeneity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Escherichia coli phosphate + ADP
-
?
YRGITCSGRQK(benzoic acid) + H2O S2 peptide Escherichia coli ?
-
?
YRGITCSGRQK-(dansyl) + H2O S4 peptide Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
lon
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
ATP wild-type Escherichia coli
0.31
-
ATP mutant S679A Escherichia coli
0.64
-
ATP mutant S679W Escherichia coli
1.07
-
ATP mutant S679W with S3 peptide Escherichia coli
1.43
-
ATP wild-type with S2 peptide Escherichia coli
1.57
-
ATP mutant S679A with S3 peptide Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP optimal peptide cleavage by lon requires hydrolysis of ATP to fully activate the proteolytic site Escherichia coli