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Literature summary for 3.4.21.53 extracted from

  • Starkova, N.N.; Koroleva, E.P.; Rumsh, L.D.; Ginodman, L.M.; Rotanova, T.V.
    Mutations in the proteolytic domain of Escherichia coli protease Lon impair the ATPase activity of the enzyme (1998), FEBS Lett., 422, 218-220.
    View publication on PubMed
Search for more literature for 3.4.21.53

Protein Variants

Protein Variants Comment Organism
D676N site-directed mutagenesis Escherichia coli
D743N site-directed mutagenesis Escherichia coli
H665Y site-directed mutagenesis Escherichia coli
H667Y site-directed mutagenesis Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 ATP pH 7.8, 37°C, wild-type Escherichia coli
0.17
-
 ATP pH 7.8, 37°C, mutant S679A Escherichia coli
0.18
-
 ATP pH 7.8, 37°C, wild-type, 0.3 mg/ml casein Escherichia coli
0.2
-
 ATP pH 7.8, 37°C, mutant S679A, 0.3 mg/ml casein Escherichia coli
0.48
-
 ATP pH 7.8, 37°C, mutant H667Y, 0.3 mg/ml casein Escherichia coli
0.5
-
 ATP pH 7.8, 37°C, mutant H667Y Escherichia coli
0.51
-
 ATP pH 7.8, 37°C, mutant H665Y, 0.3 mg/ml casein Escherichia coli
0.54
-
 ATP pH 7.8, 37°C, mutant H665Y Escherichia coli
0.56
-
 ATP pH 7.8, 37°C, mutant D676N Escherichia coli
0.58
-
 ATP pH 7.8, 37°C, mutant D676N, 0.3 mg/ml casein Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Escherichia coli phosphate + ADP
-
 ?

Synonyms

Synonyms Comment Organism
lon protease
-
 Escherichia coli
protease lon
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.031
-
 ATP pH 7.8, 37°C, mutant H667Y Escherichia coli
0.046
-
 ATP pH 7.8, 37°C, mutant H665Y Escherichia coli
0.066
-
 ATP pH 7.8, 37°C, mutant D676N Escherichia coli
0.07
-
 ATP pH 7.8, 37°C, mutant H667Y, 0.3 mg/ml casein Escherichia coli
0.073
-
 ATP pH 7.8, 37°C, mutant D676N, 0.3 mg/ml casein Escherichia coli
0.086
-
 ATP pH 7.8, 37°C, mutant H665Y, 0.3 mg/ml casein Escherichia coli
0.316
-
 ATP pH 7.8, 37°C, wild-type and mutant S679A Escherichia coli
0.65
-
 ATP pH 7.8, 37°C, mutant S679A, 0.3 mg/ml casein Escherichia coli
0.8
-
 ATP pH 7.8, 37°C, wild-type, 0.3 mg/ml casein Escherichia coli