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Literature summary for 3.4.21.53 extracted from
- Yeast mitochondrial ATP-dependent protease: purification and comparison with the homologous rat enzyme and the bacterial ATP-dependent protease La (1993), FEBS Lett., 329, 47-50.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | enzyme does not exhibit any proteolytic activity in the presence of ADP or AMPPCP, a nonhydrolyzable ATP analog | Saccharomyces cerevisiae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenyl-5'-yl methylene diphosphonate | i.e. AMP-PCP | Saccharomyces cerevisiae | |
| ADP | - |
Rattus norvegicus | |
| EDTA | 25 mM; strong | Rattus norvegicus | |
| EDTA | 25 mM; strong | Saccharomyces cerevisiae | |
| N-ethylmaleimide | - |
Rattus norvegicus | |
| N-ethylmaleimide | 1 mM, strong; high sensitivity to N-ethylmaleimide (NEM) points to an essential role of sulfhydryl groups in the enzyme activity | Saccharomyces cerevisiae | |
| PMSF | - |
Rattus norvegicus | |
| PMSF | inhibition of the yeast enzyme activity by PMSF indicates that, the enzyme is also a serine protease | Saccharomyces cerevisiae | |
| vanadate | - |
Rattus norvegicus | |
| vanadate | activity of the yeast protease is sensitive to vanadate, a potent inhibitor of many ATPases | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
ATP | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharomyces cerevisiae | |
| Mg2+ | requirement | Rattus norvegicus | |
| Mg2+ | requirement | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
6 * 105000, SDS-PAGE | Rattus norvegicus |
| 105000 | - |
6 * 105000, SDS-PAGE | Saccharomyces cerevisiae |
| 120000 | - |
purified enzyme shows a single protein band with a corresponding molecular mass of 120 kDa in SDS-PAGE, six subunits | Saccharomyces cerevisiae |
| 120000 | - |
6 * 120000, Saccharomyces cerevisiae, SDS-PAGE | Rattus norvegicus |
| 700000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae | - |
enzyme immunochemically related with Escherichia coli protease la | - |
| Saccharomyces cerevisiae | - |
yeast, mutant strain pep949 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.03559 mg casein/mg enzyme/min (Saccharomyces cerevisiae) | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Saccharomyces cerevisiae | phosphate + ADP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 105000, SDS-PAGE | Rattus norvegicus |
| hexamer | 6 * 105000, SDS-PAGE | Saccharomyces cerevisiae |
| hexamer | 6 * 120000, Saccharomyces cerevisiae, SDS-PAGE | Rattus norvegicus |
| hexamer | 6 * 120000, Saccharomyces cerevisiae, SDS-PAGE | Saccharomyces cerevisiae |
| hexamer | 6 * 120000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| protease | - |
Saccharomyces cerevisiae |
| serine protease | - |
Saccharomyces cerevisiae |
| yeast protease | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
- |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | ATP-dependent protease | Rattus norvegicus | |
| ATP | ATP-dependent protease | Saccharomyces cerevisiae | |
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