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Literature summary for 3.4.21.50 extracted from
- Evaluation of endoproteinase Lys-C/trypsin sequential digestion used in proteomics sample preparation (2017), Chin. J. Anal. Chem., 45, 316-321 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | endoproteinase lysine-C (Lys-C)/trypsin sequential digestion can be used in human 293T cell proteomics sample preparation, evaluation of the advantages of Lys-C/trypsin sequential digestion over trypsin digestion in solution conditions in different aspects, method optimzation, overview | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| enzyme Lys-C has enzymatic activity even under strong denaturing conditions, e.g. 8 M urea | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Urea | Lys-C has enzymatic activity even under strong denaturing conditions, e.g. 8 M urea | Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEK-293T cell | - |
Homo sapiens | - |
| renal epithelial cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | endoproteinase lysine-C (Lys-C) can specifically identify and cleave C-terminal lysine with high efficiency and specificity | Homo sapiens | ? | - |
? | |
| additional information | endoproteinase lysine-C (Lys-C)/trypsin sequential digestion can be used in human 293T cell proteomics sample preparation, evaluation of the advantages of Lys-C/trypsin sequential digestion over trypsin digestion in solution conditions in different aspects, method optimzation, 2 M urea and trypsin in 50:1 or 100:1 ratio, overview. The peptides obtained by sequential digestion are of proper length and can be easily extracted from the gel so as to be more suitable for mass spectrometric identification | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoproteinase lysine-C | - |
Homo sapiens |
| Lys-C | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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