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Literature summary for 3.4.21.50 extracted from

  • Shiraki, K.; Norioka, S.; Li, S.; Sakiyama, F.
    Contribution of an imidazole-indole stack to high catalytic potency of a lysine-specific serine protease, Achromobacter protease I (2002), J. Biochem., 131, 213-218.
    View publication on PubMed
Search for more literature for 3.4.21.50

Protein Variants

Protein Variants Comment Organism
H210A the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin is 7.1% of the wild-type value, the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is identical to the wild-type value Achromobacter lyticus
H210F the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin is 14% of the wild-type value, the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 29.5% of the wild-type value Achromobacter lyticus
H210S the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin is 8.6% of the wild-type value, the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 1.7fold higher than the wild-type value Achromobacter lyticus
W169A the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 0.5% of the wild-type value Achromobacter lyticus
W169F the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 45% of the wild-type value Achromobacter lyticus
W169G the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 0.06% of the wild-type value Achromobacter lyticus
W169H the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 9% of the wild-type value Achromobacter lyticus
W169L the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 25% of the wild-type value Achromobacter lyticus
W169V the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 6% of the wild-type value Achromobacter lyticus
W169Y the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 43% of the wild-type value Achromobacter lyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210S Achromobacter lyticus
0.0013
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
0.0021
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
0.0028
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
0.0034
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
0.0036
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
0.0037
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
0.004
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
0.0043
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
0.0046
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
0.0058
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
0.006
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210S Achromobacter lyticus
0.0061
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
0.0063
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
0.0072
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
0.0073
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
0.0099
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
0.01
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
0.01
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
0.012
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
0.017
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
0.02
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
0.023
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
0.024
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
0.029
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
0.032
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169G Achromobacter lyticus
0.034
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
0.034
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
0.037
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
0.038
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
0.042
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
0.046
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
0.066
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
0.074
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
0.081
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus

Organism

Organism UniProt Comment Textmining
Achromobacter lyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin + H2O
-
 Achromobacter lyticus tert-butyloxycarbonyl-Ala-Ala-Lys + 7-amino-4-methylcoumarin
-
 ?
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin + H2O
-
 Achromobacter lyticus tert-butyloxycarbonyl-Ala-Lys + 7-amino-4-methylcoumarin
-
 ?
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin + H2O
-
 Achromobacter lyticus tert-butyloxycarbonyl-Lys + 7-amino-4-methylcoumarin
-
 ?
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
 Achromobacter lyticus tert-butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
0.8
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169G Achromobacter lyticus
1.1
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
2 8 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
2.3
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
2.6
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
2.7
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
3 6 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
4.5
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
4.6
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A Achromobacter lyticus
5.2
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
6
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
7
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
8.4
-
 tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
11
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
15
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
18
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210S Achromobacter lyticus
20
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V Achromobacter lyticus
22
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
23
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
24
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
31
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
37
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L Achromobacter lyticus
40
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
45
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
46
-
 tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
48
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
51
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
52
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210S Achromobacter lyticus
67
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H Achromobacter lyticus
71
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F Achromobacter lyticus
83
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y Achromobacter lyticus
93
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus
96
-
 tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A Achromobacter lyticus
98
-
 tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme Achromobacter lyticus