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Literature summary for 3.4.21.50 extracted from
- Lysyl endopeptidase of Achromobacter lyticus (1994), Methods Enzymol., 244, 126-137.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the cloned gene of prepro-API is expressed in Escherichia coli, pro-API is secreted into the periplasm and activated autocatalytically | Achromobacter lyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-amino-1-hexanoic acid | - |
Achromobacter lyticus |  |
| acetonitrile | - |
Achromobacter lyticus | |
| Ba2+ | - |
Achromobacter lyticus | |
| benzoyl-DL-lysinal | - |
Achromobacter lyticus | |
| Benzoyl-Lys | - |
Achromobacter lyticus | |
| benzyloxycarbonyl-Leu-Leu-lysinal | - |
Achromobacter lyticus | |
| benzyloxycarbonyl-Pro-lysinal | - |
Achromobacter lyticus | |
| benzyloxycarbonyl-Val-lysinal | - |
Achromobacter lyticus | |
| Cs+ | - |
Achromobacter lyticus | |
| Cyclohexylamine | - |
Achromobacter lyticus | |
| diisopropylfluorophosphate | - |
Achromobacter lyticus | |
| ethylamine | - |
Achromobacter lyticus | |
| hydrazine | - |
Achromobacter lyticus | |
| Isobutylamine | - |
Achromobacter lyticus | |
| K+ | - |
Achromobacter lyticus | |
| Li+ | - |
Achromobacter lyticus | |
| Lys | - |
Achromobacter lyticus | |
| methylamine | - |
Achromobacter lyticus | |
| n-alkylamine | - |
Achromobacter lyticus | |
| n-amylamine | - |
Achromobacter lyticus | |
| n-butylamine | - |
Achromobacter lyticus | |
| n-Hexylamine | - |
Achromobacter lyticus | |
| n-Propylamine | - |
Achromobacter lyticus | |
| Na+ | - |
Achromobacter lyticus | |
| Nalpha-p-tosyl-L-Lys-chloromethylketone | - |
Achromobacter lyticus | |
| NH4+ | - |
Achromobacter lyticus | |
| phenylmethanesulfonyl fluoride | - |
Achromobacter lyticus | |
| Rb+ | - |
Achromobacter lyticus | |
| Zn2+ | - |
Achromobacter lyticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
Trypsinogen | - |
Achromobacter lyticus | |
| 0.07 | - |
N-benzoyl-L-Lys p-nitroanilide | - |
Achromobacter lyticus | |
| 0.08 | - |
Lys-p-nitroanilide | - |
Achromobacter lyticus | |
| 0.091 | - |
benzoyl-Lys methyl ester | - |
Achromobacter lyticus | |
| 0.1 | - |
N-tosyl-L-Lys methyl ester | - |
Achromobacter lyticus | |
| 0.32 | - |
benzoyl-Lys-NH2 | - |
Achromobacter lyticus | |
| 1 | - |
N-benzoyl-L-Orn methyl ester | - |
Achromobacter lyticus | |
| 3.09 | - |
N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
| 3.11 | - |
N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
| 35.7 | - |
N-tosyl-L-Arg methyl ester | - |
Achromobacter lyticus | |
| 41 | - |
N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-propanol | 20%, 37°C, enzyme retains most of its activity | Achromobacter lyticus |
| Ethanol | 20%, 37°C, enzyme retains most of its activity | Achromobacter lyticus |
| Methanol | 20%, 37°C, enzyme retains most of its activity | Achromobacter lyticus |
| SDS | 0.1% w/v, in 40 mM Tris-HCl, pH 8, 20 min, 30°C, stable | Achromobacter lyticus |
| urea | 4 M, stable | Achromobacter lyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter lyticus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the cloned gene of prepro-API is expressed in E. coli, pro-API is secreted into the periplasm and activated autocatalytically | Achromobacter lyticus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Achromobacter lyticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Achromobacter lyticus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.001-0.1 M Tris-HCl buffer, pH 6.0-10.9, at enzyme concentration higher than 0.1 mg/ml | Achromobacter lyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-Lys methyl ester + H2O | - |
Achromobacter lyticus | benzoyl-Lys + methanol | - |
? | |
| benzoyl-Lys-NH2 + H2O | - |
Achromobacter lyticus | benzoyl-Lys + NH3 | - |
? | |
| benzoyl-Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus | benzoyl-Lys + p-nitroaniline | - |
? | |
| Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus | Lys + p-nitroaniline | - |
? | |
| N-benzoyl-L-Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus | N-benzoyl-L-Lys + p-nitroaniline | - |
? | |
| N-benzoyl-L-Orn methyl ester + H2O | - |
Achromobacter lyticus | N-benzoyl-L-Orn + methanol | - |
? | |
| N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide + H2O | - |
Achromobacter lyticus | N-tert-butyloxycarbonyl-Ala-Ala-Lys + 7-amino-4-methylcoumarin | - |
? | |
| N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide + H2O | - |
Achromobacter lyticus | N-tert-butyloxycarbonyl-Ala-Lys + 7-amino-4-methylcoumarin | - |
? | |
| N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide + H2O | - |
Achromobacter lyticus | N-tert-butyloxycarbonyl-Lys + 7-amino-4-methylcoumarin | - |
? | |
| N-tosyl-L-Arg methyl ester + H2O | - |
Achromobacter lyticus | N-tosyl-L-Arg + methanol | - |
? | |
| N-tosyl-L-Lys methyl ester + H2O | - |
Achromobacter lyticus | N-tosyl-L-Lys + methanol | - |
? | |
| Trypsinogen + H2O | - |
Achromobacter lyticus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable | Achromobacter lyticus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.103 | - |
Trypsinogen | - |
Achromobacter lyticus | |
| 0.14 | - |
Lys-p-nitroanilide | - |
Achromobacter lyticus | |
| 0.86 | - |
benzoyl-Lys-p-nitroanilide | - |
Achromobacter lyticus | |
| 1.54 | - |
benzoyl-Lys-NH2 | - |
Achromobacter lyticus | |
| 3.02 | - |
N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
| 5.04 | - |
N-tosyl-L-Arg methyl ester | - |
Achromobacter lyticus | |
| 10.4 | - |
N-benzoyl-L-Orn methyl ester | - |
Achromobacter lyticus | |
| 32.9 | - |
N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
| 49 | - |
N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide | - |
Achromobacter lyticus | |
| 225 | - |
benzoyl-Lys methyl ester | - |
Achromobacter lyticus | |
| 570 | - |
N-tosyl-L-Lys methyl ester | - |
Achromobacter lyticus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.2 | - |
Achromobacter lyticus |
| 8.5 | 10.7 | caseinolytic activity | Achromobacter lyticus |
| 9 | 9.5 | - |
Achromobacter lyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | stable | Achromobacter lyticus |
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