Any feedback?
Literature summary for 3.4.21.50 extracted from
- Studies on a new proteolytic enzyme from A chromobacter lyticus M497-1. I. Purification and some enzymatic properties (1981), Biochim. Biophys. Acta, 660, 44-50.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | competitive | Achromobacter lyticus |  |
| Cs+ | competitive | Achromobacter lyticus | |
| diisopropylfluorophosphate | - |
Achromobacter lyticus | |
| K+ | competitive | Achromobacter lyticus | |
| Li+ | competitive | Achromobacter lyticus | |
| Na+ | competitive | Achromobacter lyticus | |
| Nalpha-p-tosyl-L-Lys-chloromethylketone | - |
Achromobacter lyticus | |
| NH4+ | competitive | Achromobacter lyticus | |
| phenylmethanesulfonyl fluoride | - |
Achromobacter lyticus | |
| Rb+ | competitive | Achromobacter lyticus | |
| Zn2+ | - |
Achromobacter lyticus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Achromobacter lyticus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
1 * 30000, SDS-PAGE | Achromobacter lyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter lyticus | - |
M497-1 | - |
| Achromobacter lyticus M497-1 | - |
M497-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Achromobacter lyticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Achromobacter lyticus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Achromobacter lyticus | ? | - |
? | |
| casein + H2O | - |
Achromobacter lyticus M497-1 | ? | - |
? | |
| additional information | the enzyme cleaves only the Lys-X bond | Achromobacter lyticus | ? | - |
? | |
| additional information | the enzyme cleaves only the Lys-X bond | Achromobacter lyticus M497-1 | ? | - |
? | |
| N-benzoyl-L-Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus | N-benzoyl-L-Lys + p-nitroaniline | - |
? | |
| N-benzoyl-L-Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus M497-1 | N-benzoyl-L-Lys + p-nitroaniline | - |
? | |
| N-tosyl-L-Lys methyl ester + H2O | - |
Achromobacter lyticus | N-tosyl-L-Lys + methanol | - |
? | |
| N-tosyl-L-Lys methyl ester + H2O | - |
Achromobacter lyticus M497-1 | N-tosyl-L-Lys + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE | Achromobacter lyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
amidolytic activity, esterolytic activity and caseinolytic activity | Achromobacter lyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
pH 9.0, 30 min, stable up to | Achromobacter lyticus |
| 60 | - |
30 min, complete loss of activity | Achromobacter lyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.2 | esterolytic activity | Achromobacter lyticus |
| 7.8 | 8.2 | esterolytic activity | Achromobacter lyticus |
| 9 | 9.5 | amidolytic activity | Achromobacter lyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | 4°C, 24 h, stable | Achromobacter lyticus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
