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Literature summary for 3.4.21.5 extracted from

  • Fenton, J.W.; Fasco, M.J.; Stackrow, A.B.; Aronson, D.L.; Young, A.M.; Finlayson, J.S
    Human thrombins. Production, evaluation, and properties of alpha-thrombin (1977), J. Biol. Chem., 252, 3587-3598.
    View publication on PubMed

General Stability

General Stability Organism
autolytic degradation during sedimentation in neutral salt solution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31500 40600 thrombin in 0.21 M NaCl, 0.02 M sodium phosphate, pH 7.2, equilibrium sedimentation Homo sapiens
32000
-
1 * 4600, A chain, + 1 * 32000, B chain, reduced alpha-thrombin, SDS-PAGE Homo sapiens
36600
-
equilibrium sedimentation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification carbohydrate is associated only with the B chain Homo sapiens

Storage Stability

Storage Stability Organism
-50°C, 0.75 M NaCl, pH 6, stable for about 1 year Homo sapiens
4°C, 0.75 M NaCl, pH 6, stable for about 1 week Homo sapiens

Subunits

Subunits Comment Organism
dimer 1 * 4600, A chain, + 1 * 32000, B chain, reduced alpha-thrombin, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
23
-
stable for up to 3 days at room temperature Homo sapiens
35
-
pH 6, loss of activity above Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10.3 rapid loss of clotting and esterase activity below pH 5 and above pH 10.3 Homo sapiens