Application | Comment | Organism |
---|---|---|
medicine | fibrinogenolytic properties make the enzyme applicable for biochemical research and drug development on thrombolytic therapy | Deinagkistrodon acutus |
Cloned (Comment) | Organism |
---|---|
a recombinant enzyme expression vector is constructed and the fused protein is expressed in Escherichia coli | Deinagkistrodon acutus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by EDTA | Deinagkistrodon acutus | |
PMSF | 2 mM, complete inhibition | Deinagkistrodon acutus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49400 | - |
SDS-PAGE, recombinant enzyme containing the 20400 Da Trx tag | Deinagkistrodon acutus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinagkistrodon acutus | Q5I2C5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Deinagkistrodon acutus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Deinagkistrodon acutus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrinogen Aalpha-chain + H2O | - |
Deinagkistrodon acutus | ? | - |
? | |
additional information | the enzyme shows no factor XIII activation activity | Deinagkistrodon acutus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49400, SDS-PAGE, recombinant enzyme containing the 20400 Da Trx tag | Deinagkistrodon acutus |
Synonyms | Comment | Organism |
---|---|---|
TLE2 | - |
Deinagkistrodon acutus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Deinagkistrodon acutus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | 15°C: about 45% of maximal activity, 50°C: about 50% of maximal activity | Deinagkistrodon acutus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Deinagkistrodon acutus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | pH 5.5: about 60% of maximal activity, pH 9.0: about 60% of maximal activity | Deinagkistrodon acutus |