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Literature summary for 3.4.21.5 extracted from

  • Liu, Y.; Liu, N.; Ma, X.; Li, X.; Ma, J.; Li, Y.; Zhou, Z.; Gao, Z.
    Highly specific detection of thrombin using an aptamer-based suspension array and the interaction analysis via microscale thermophoresis (2015), Analyst, 140, 2762.
    View publication on PubMed

Application

Application Comment Organism
analysis design of an aptamer-based suspension array detection platform for the sensitive, specific and rapid detection of human alpha-thrombin as a model. Thrombin is first recognized by a 29-mer biotinylated thrombin-binding aptamer in solution. Then 15-mer TBA modified magnetic beads capture the former aptamer-thrombin to form an aptamer-thrombin-aptamer sandwich complex. The median fluorescence intensity obtained via suspension array technology is positively correlated with the thrombin concentration. The dynamic quantitative working range of the aptamer-based suspension array is 18.37-554.31 nM, and the coefficients of determination R2 are greater than 0.9975. The lowest detection limit of thrombin is 5.4 nM. The method is highly specific for thrombin without being affected by other analogs and interfering proteins. The recoveries of thrombin spiked in diluted human serum are in the range 82.6-114.2% Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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Homo sapiens
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