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Literature summary for 3.4.21.5 extracted from

  • Isetti, G.; Maurer, M.C.
    Employing mutants to study thrombin residues responsible for factor XIII activation peptide recognition: a kinetic study (2007), Biochemistry, 46, 2444-2452.
    View publication on PubMed
Search for more literature for 3.4.21.5

Protein Variants

Protein Variants Comment Organism
E217A site-directed mutagenesis, mutation of a residue involved in interaction with substrate factore XIII, the mutant shows reduced activity compared to the wild-type enzyme, the mutation affects hydrolysis of substrates due to interruption of key structural contacts involving the thrombin 220 loop and the S1 specificity pocket Homo sapiens
I174A site-directed mutagenesis, mutation of a residue involved in interaction with substrate factore XIII, the mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
L99A site-directed mutagenesis, mutation of a residue involved in interaction with substrate factore XIII, the mutant shows eliminated activity compared to the wild-type enzyme Homo sapiens
W215A site-directed mutagenesis, mutation of a residue involved in interaction with substrate factore XIII, the mutant shows highly reduced activity compared to the wild-type enzyme Homo sapiens
W215A/E217A site-directed mutagenesis, mutation of a residue involved in interaction with substrate factore XIII, the mutant shows highly reduced activity compared to the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of mutant enzymes, overview Homo sapiens
0.298
-
 factor XIII pH 7.4, 25°C, wild-type enzyme Homo sapiens
0.315
-
 factor XIII V34L mutant pH 7.4, 25°C, wild-type enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Na+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
factor XIII + H2O Homo sapiens the enzyme is involved in the coagulation cascade, overview activated factor XIII + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor XIII + H2O the enzyme is involved in the coagulation cascade, overview Homo sapiens activated factor XIII + ?
-
 ?
factor XIII + H2O binding structure and interaction analysis, wild-type substrate, overview Homo sapiens activated factor XIII + ?
-
 ?
factor XIII V34L mutant + H2O binding structure and interaction analysis, mutant substrate, a polymorphism exists within the activation peptide segment at the P4 position of FXIII resulting in substitution V34L, FXIII V34L occurs in approximately 30% of the human population worldwide, overview Homo sapiens activated factor XIII V34L mutant + ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.57
-
 factor XIII pH 7.4, 25°C, wild-type enzyme Homo sapiens
22.9
-
 factor XIII V34L mutant pH 7.4, 25°C, wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens