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Literature summary for 3.4.21.49 extracted from
- Immunosuppressive effect of hypodermin C on complement component 3 in vitro (2015), Cell Biochem. Biophys., 72, 93-98.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, recombinant expression in COS7 cells using the pEF1a-IRES-AcGFP expression vector. COS7 cells stably expressing the enzyme are more resistant to lysis by guinea pig complement C3 than the control cells | Hypoderma lineatum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted by the first-instar larvae | Hypoderma lineatum | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hypoderma lineatum | P08897 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| larva | the enzyme is secreted by the first-instar larvae | Hypoderma lineatum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guinea pig complement C3 + H2O | recombinant enzyme, degradation | Hypoderma lineatum | ? | - |
? |  |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Hypoderma lineatum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Hypoderma lineatum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | identification of the DNA binding sites of the enzyme using an electrophoretic mobility shift asay | Hypoderma lineatum |
| physiological function | hypodermin C protease degrades the guinea pig complement C3, and inhibits the complement pathway in vitro, immunosuppressive effect of hypodermin C on complement component 3 in vitro, the cytotoxicity of C3 is inhibited in cultures of stably transfected COS7 cells expressing the enzyme | Hypoderma lineatum |
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