BRENDA - Enzyme Database show
show all sequences of 3.4.21.48

Purification and properties of proteinase B from yeast

Kominami, E.; Hoffschulte, H.; Holzer, H.; Biochim. Biophys. Acta 661, 124-135 (1981)

Data extracted from this reference:

General Stability
General Stability
Organism
freezing and thawing in absence of glycerol results in total loss of activity
Saccharomyces cerevisiae
HgCl2, 10 mM, stabilizes during purification
Saccharomyces cerevisiae
Inhibitors
Inhibitors
Commentary
Organism
Structure
antipain
-
Saccharomyces cerevisiae
Aprotinin
i.e. trasylol, weak
Saccharomyces cerevisiae
chymostatin
-
Saccharomyces cerevisiae
DFP
-
Saccharomyces cerevisiae
Hg2+
HgCl2
Saccharomyces cerevisiae
p-hydroxymercuribenzoate
-
Saccharomyces cerevisiae
PMSF
-
Saccharomyces cerevisiae
Trypsin inhibitor
weak
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
vacuole
-
Saccharomyces cerevisiae
5773
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, SDS-PAGE
Saccharomyces cerevisiae
33700
-
equilibroum sedimentation
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
Saccharomyces carlsbergensis; Saccharomyces cerevisiae
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
side-chain modification
contains 8-9% neutral sugars and 1.5% amino sugars
Saccharomyces cerevisiae
Purification (Commentary)
Commentary
Organism
large scale
Saccharomyces cerevisiae
Storage Stability
Storage Stability
Organism
-20°C, 20% glycerol, 0.1 mM HgCl2, stable for 2 months
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Azocoll + H2O
-
95499
Saccharomyces cerevisiae
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 33000, SDS-PAGE
Saccharomyces cerevisiae
General Stability (protein specific)
General Stability
Organism
freezing and thawing in absence of glycerol results in total loss of activity
Saccharomyces cerevisiae
HgCl2, 10 mM, stabilizes during purification
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
antipain
-
Saccharomyces cerevisiae
Aprotinin
i.e. trasylol, weak
Saccharomyces cerevisiae
chymostatin
-
Saccharomyces cerevisiae
DFP
-
Saccharomyces cerevisiae
Hg2+
HgCl2
Saccharomyces cerevisiae
p-hydroxymercuribenzoate
-
Saccharomyces cerevisiae
PMSF
-
Saccharomyces cerevisiae
Trypsin inhibitor
weak
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
vacuole
-
Saccharomyces cerevisiae
5773
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, SDS-PAGE
Saccharomyces cerevisiae
33700
-
equilibroum sedimentation
Saccharomyces cerevisiae
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
side-chain modification
contains 8-9% neutral sugars and 1.5% amino sugars
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
large scale
Saccharomyces cerevisiae
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 20% glycerol, 0.1 mM HgCl2, stable for 2 months
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Azocoll + H2O
-
95499
Saccharomyces cerevisiae
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 33000, SDS-PAGE
Saccharomyces cerevisiae
Other publictions for EC 3.4.21.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718011
Morozkina
Proteinase B disruption is req ...
Saccharomyces cerevisiae
J. Mol. Microbiol. Biotechnol.
18
188-194
2010
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718456
Perez
Upregulation of the PRB1 gene ...
Saccharomyces cerevisiae
Yeast
27
575-581
2010
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1
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1
1
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682540
Subramanian
Pbn1p: an essential endoplasmi ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BJ 5410
Proc. Natl. Acad. Sci. USA
103
939-944
2006
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683085
Kato
Analysis of a processing syste ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae MT8-1
Appl. Microbiol. Biotechnol.
72
1229-1237
2006
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1
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668984
Bolumar
Protease B from Debaryomyces h ...
Debaryomyces hansenii
Int. J. Food Microbiol.
98
167-177
2005
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2
1
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1
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1
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1
1
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95510
Rupp
Biogenesis of the yeast vacuol ...
Saccharomyces cerevisiae
Eur. J. Biochem.
231
115-125
1995
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1
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95513
Campbell
-
Inhibition of proteinase B and ...
Saccharomyces cerevisiae
J. Gen. Appl. Microbiol.
41
449-453
1995
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1
1
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1
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95509
Hirsch.H.H.; Schiffer
Biogenesis of the yeast vacuol ...
Saccharomyces cerevisiae
Eur. J. Biochem.
203
641-653
1992
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1
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2
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95512
Larrinoa
Yeast proteinase yscb inactiva ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1073
502-508
1991
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1
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95515
Nebes
Activation of the proteinase B ...
Saccharomyces cerevisiae
J. Biol. Chem.
266
22851-22857
1991
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30710
Nowak
Purification and properties of ...
Saccharomyces cerevisiae
Can. J. Microbiol.
35
295-303
1989
1
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3
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1
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1
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95514
Moehle
Protease B of the lysosomelike ...
Saccharomyces cerevisiae
Mol. Cell. Biol.
7
4390-4399
1987
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95499
Kominami
Purification and properties of ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
661
124-135
1981
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2
8
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1
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2
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1
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8
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1
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95500
Kominami
The substrate specificity of p ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
661
136-141
1981
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2
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1
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7
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7
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2
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95507
Schwencke
Measurement of proteinase B ac ...
Saccharomyces cerevisiae
Anal. Biochem.
118
315-321
1981
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1
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95501
Fujishiro
Purification and characterizat ...
Saccharomyces cerevisiae
J. Biochem.
87
1321-1326
1980
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9
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4
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9
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4
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95502
Maier
Purification and molecular cha ...
Saccharomyces cerevisiae
J. Biol. Chem.
254
8491-8497
1979
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95503
Looze
Protease B from Saccharomyces ...
Saccharomyces cerevisiae
Int. J. Pept. Protein Res.
13
253-259
1979
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95504
Sanada
Isolation and characterization ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
86
815-821
1979
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3
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95505
Holzer
Chemistry and biology of macro ...
Saccharomyces cerevisiae
Adv. Enzyme Regul.
13
125-134
1975
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95508
Lenney
Purification and properties of ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
120
42-48
1967
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1
4
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1
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4
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1
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1
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95506
Felix
Purification et proprietes de ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
122
127-144
1966
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3
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3
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7
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