Cloned (Comment) | Organism |
---|---|
generation of a yeast strain with PBN1 under control of the GAL promoter and of strains depleted of Pbn1p, transformation of Saccaromyces BJ 5410 with the PBN1 gene to generate the pGAL-HA-PBN1 strain BJ 10242, properties of strain BJ 10242 by processing activities of transient proteins of the endoplasmic reticulum indicated | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | membrane, type I glycoprotein | Saccharomyces cerevisiae | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P25580 | Pbn1p, encoded by the PBN1 gene; BJ 5410 | - |
Saccharomyces cerevisiae BJ 5410 | P25580 | Pbn1p, encoded by the PBN1 gene; BJ 5410 | - |
Purification (Comment) | Organism |
---|---|
gel filtration | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
processing activities of transient proteins of the endoplasmic reticulum indicated, depletion of Pbn1p abrogates processing of precursor proteins of the endoplasmic reticulum but not global exit from the endoplasmic reticulum, significant increase in the unfolded protein response pathway observed indicating defects in protein folding in the endoplasmic reticulum | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pbn1p shown as an essential chaperone-like protein in the endoplasmic reticulum of yeast, essential function of the Pbn1 protein for protein processing in the membrane of the endoplasmic reticulum described, requirement for proper folding and stability of a subset of proteins in the endoplasmic reticulum indicated | Saccharomyces cerevisiae | ? | - |
? | |
additional information | Pbn1p shown as an essential chaperone-like protein in the endoplasmic reticulum of yeast, essential function of the Pbn1 protein for protein processing in the membrane of the endoplasmic reticulum described, requirement for proper folding and stability of a subset of proteins in the endoplasmic reticulum indicated | Saccharomyces cerevisiae BJ 5410 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Proteinase B | - |
Saccharomyces cerevisiae |