Activating Compound | Comment | Organism | Structure |
---|---|---|---|
properdin | positive regulator of the alternative pathway, no direct interaction with the decay-accelerating factor DAF determined | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
decay-accelerating factor | DAF, CD55, major site of interaction with the larger cleavage subunit complement component factor B (Bb), interaction pathway dissected, second short consensus repeat (SCR) domain of DAF (SCR2) interacts only with fragment Bb, whereas SCR4 interacts with complement component C3b, SCR3 does not directly interact with either subunit | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement component C3 + H2O | Homo sapiens | - |
complement component C3a + complement component C3b | - |
? | |
complement component C5 + H2O | Homo sapiens | - |
complement component C5a + complement component C5b | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00751 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
serum | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
hemolysis assay to assess inhibition by DAF mutants described, equilibrium analysis of recombinant soluble DAF mutants binding to complement component C3b shown, kinetic constants for binding of DAF mutants to the complement component C3b and fragment Bb and ability to decay AP convertase determined, interaction of DAF with properdin and decay of properdin-stabilized convertase indicated, binding kinetics of fragment Bb to recombinant soluble DAF mutants DAF1234, DAF23 and DAF34 determined, decay of AP C3 convertase by DAF and DAF mutants monitored | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement component C3 + H2O | - |
Homo sapiens | complement component C3a + complement component C3b | - |
? | |
complement component C3 + H2O | interaction studies with decay-accelerating factor DAF mediating decay of the alternative pathway C3 convertase, C3bBb, but not of the inactive proenzyme C3bB | Homo sapiens | complement component C3a + complement component C3b | - |
? | |
complement component C5 + H2O | - |
Homo sapiens | complement component C5a + complement component C5b | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C3 convertase | - |
Homo sapiens |