Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.45 extracted from

  • Crossley, L.G.
    C3b inactivator and beta1H (1981), Methods Enzymol., 80, 112-124.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
beta1H potentiates the action of the enzyme on C3b Homo sapiens
C4-binding protein cofactor Homo sapiens

General Stability

General Stability Organism
stable to 0.15 M hydrazine or 2 M guanidine-HCl Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 10 mM, strong Homo sapiens
dithiothreitol 1 mM, strong Homo sapiens
NEM 1 mM, partial inhibition Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
1 * 38000 + 1 * 50000, non-catalytic 50000 Da subunit is disulfide-linked to the 38000 Da catalytic subunit Homo sapiens
50000
-
1 * 38000 + 1 * 50000, non-catalytic 50000 Da subunit is disulfide-linked to the 38000 Da catalytic subunit Homo sapiens
88000
-
sucrose density gradient centrifugation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification carbohydrate content is at least 10.7% w/w, not including neuraminic acid, with 7.2% hexose and 3.2% glucosamine Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
Homo sapiens
-
serum
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
4°C, neutral pH, stable for several weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C3b + H2O
-
Homo sapiens complement component C3c + ?
-
?
complement component C4b + H2O
-
Homo sapiens complement component C4c + C4d
-
?

Subunits

Subunits Comment Organism
dimer 1 * 38000 + 1 * 50000, non-catalytic 50000 Da subunit is disulfide-linked to the 38000 Da catalytic subunit Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
30 min, stable Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.2
-
stable Homo sapiens