Application | Comment | Organism |
---|---|---|
medicine | studies on mutations of complement factor I | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, mutant and unmodified proteins | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G243D | cofactor function of complement components C3b and Cb4 not affected | Homo sapiens |
M120I | clinical data of aHUS-patients with complemment factor I mutation summarized, cofactor function of complement components C3b and Cb4 not affected | Homo sapiens |
R388H | cofactor function of complement components C3b and Cb4 not affected | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
heavy chain, indicative of processing | Homo sapiens |
88000 | - |
non-reduced form, SDS-PAGE | Homo sapiens |
88000 | - |
reduced form, RRKR-linker peptide not cleaved, corresponds to pro-complemement factor I | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement component C3b + H2O | Homo sapiens | - |
complement component C3c + ? | - |
? | |
complement component C4b + H2O | Homo sapiens | - |
complement component C4c + C4d | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P05156 | encoded by CFI gene | - |
Purification (Comment) | Organism |
---|---|
recombinant and native proteins, gel filtration, SDS-PAGE | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
keratinocyte | incubation of foreskin with thermolysin followed by trypsinization | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
keratinocytes stimulated with IFN-gamma (10-1000 U/ml), IL-1alpha (25-200 U/ml), IL-6 (250-1000 U/ml), TNF-alpha (100-1000 U/ml) or TGF-beta1, presence of complement factor I analyzed by monitoring cleavage of complement component C3b, assay with radiolabelled purified complement component C3b | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement component C3b + H2O | - |
Homo sapiens | complement component C3c + ? | - |
? | |
complement component C3b + H2O | complement factor I production of human keratinocytes analyzed, with and without stimulation by IFN-gamma, IL-1alpha, IL-6, TNF-alpha, TGF-beta1 | Homo sapiens | complement component C3c + ? | - |
? | |
complement component C4b + H2O | - |
Homo sapiens | complement component C4c + C4d | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
C3b cleavage assay at | Homo sapiens |