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Literature summary for 3.4.21.42 extracted from
- Antibody-mediates inhibition of human C1s and the classical complement pathway (2013), Immunobiology, 218, 1041-1048.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | inhibition of the complement pathway is of great therapeutic interest. The C1 complex is a very attractive target for selective inhibition of the classical complement pathway because it is the only member of the classical pathway that does not participate in the other complement pathways | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of N-terminally His6-tagged enzyme fragment comprising the second CCP domain and the serine protease domain, CCP2-SP, amino acids 358-688 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | engineering of a potenthuman antibody inhibitor of C1s protease activity, inhibition of recombinnat enzyme fragment CCP2-SP, overview. The C1 complex is a very attractive target for selective inhibition of the classical complement pathway because it is the only member of the classical pathway that does not participate in the other complement pathways | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Homo sapiens | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| complement C4 zymogen + H2O | Homo sapiens | - |
active complement C4 + ? | - |
? |  |
| complement C4-bound C2 zymogen + H2O | Homo sapiens | - |
C4a + C2b | the fragments associate to form a C3 convertase enzyme | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P09871 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | activation of protease C1s through cleavage by protease C1r, EC 3.4.21.41 | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme fragment CCP2 and SP by nickel affinity chromatography and dialysis, activation by C1r | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood serum | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| complement C4 zymogen + H2O | - |
Homo sapiens | active complement C4 + ? | - |
? | |
| complement C4-bound C2 zymogen + H2O | - |
Homo sapiens | C4a + C2b | the fragments associate to form a C3 convertase enzyme | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C1s protease | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | In the classical complement pathway, immune complexes formed by IgM, IgG1, IgG2 and IgG3 antibodies result in the activation of the C1s protease that in turn cleaves C4 and then C4-bound-C2 yielding the proteolytic fragments C4b and C2a which associate to form a C3 convertase enzyme | Homo sapiens |
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Release 2023.1 (January 2023)
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