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Literature summary for 3.4.21.42 extracted from

  • Wijeyewickrema, L.C.; Duncan, R.C.; Pike, R.N.
    The role of the lys628 (192) residue of the complement protease, C1s, in interacting with peptide and protein substrates (2014), Front. Immunol., 5, 444.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.42

Activating Compound

Activating Compound Comment Organism Structure
additional information the zymogen forms of the C1s protease is activated using immobilized recombinant protease C1r Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type enzyme and K628Q and K628A mutants in Escherichia coli as insoluble proteins in inclusion bodies Homo sapiens

Protein Variants

Protein Variants Comment Organism
K628A the mutant shows lower activity than wild-type enzyme against peptide substrates, cleavage of C4 is reduced by 5fold compared to the wild-type enzyme Homo sapiens
K628Q the mutant shows greater activity than wild-type enzyme against peptide substrates and peptide substrates containing physiological substrate sequences, unaltered activity with 2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 substrate compared to the wild-type enzyme, but cleavage of C4 complement is reduced by 5fold compared to the wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
C1-inhibitor
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 pH 7.4, 37°C, recombinant mutant K628A Homo sapiens
0.0123
-
 2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 pH 7.4, 37°C, recombinant mutant K628Q Homo sapiens
0.0138
-
 2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
0.0654
-
 Z-AGLQR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant mutant K628Q Homo sapiens
0.0762
-
 Z-LGR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant mutant K628Q Homo sapiens
0.109
-
 Z-AGLQR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant mutant K628A Homo sapiens
0.169
-
 Z-LGR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
0.212
-
 Z-LGR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant mutant K628A Homo sapiens
0.245
-
 Z-AGLQR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P09871
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the zymogen form of the C1s protease is activated using immobilized recombinant protease C1r Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant refolded and solubilized wild-type enzyme and K628Q and K628A mutants from Escherichia coli by anion exchange chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 + H2O the Abz-GLQRALEI-Lys(Dnp)fluorescence quenched peptide representing the P4-P4' sequence of complement C4 Homo sapiens ?
-
 ?
C4 complement + H2O
-
 Homo sapiens ?
-
 ?
additional information substrate specificity, library screening, overview. The Lys residue found at the P1' position in C2 and the Gln residue found at the P2 position of C4 are likely to be prohibitive for cleavage of substrates by the C1s protease. The K628Q and K628A mutants of C1s also do not cleave these substrate pools, indicating that K628 does not play a crucial role in preventing interaction with such substrates Homo sapiens ?
-
 ?
Z-AGLQR-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Z-AGLQR + 7-amino-4-methylcoumarin
-
 ?
Z-LGR-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Z-LGR + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
C1s
-
 Homo sapiens
C1s protease
-
 Homo sapiens
complement protease
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14
-
 2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
1.17
-
 2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 pH 7.4, 37°C, recombinant mutant K628Q Homo sapiens
1.3
-
 Z-AGLQR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant mutant K628A Homo sapiens
1.3
-
 2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 pH 7.4, 37°C, recombinant mutant K628A Homo sapiens
1.77
-
 Z-LGR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant mutant K628A Homo sapiens
2.72
-
 Z-LGR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
6
-
 Z-LGR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant mutant K628Q Homo sapiens
35.3
-
 Z-AGLQR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
116.5
-
 Z-AGLQR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant mutant K628Q Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.3
-
 Z-LGR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant mutant K628A Homo sapiens
12
-
 Z-AGLQR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant mutant K628A Homo sapiens
16
-
 Z-LGR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
79
-
 Z-LGR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant mutant K628Q Homo sapiens
82
-
 2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
95
-
 2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 pH 7.4, 37°C, recombinant mutant K628Q Homo sapiens
110
-
 2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 pH 7.4, 37°C, recombinant mutant K628A Homo sapiens
140
-
 Z-AGLQR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
1800
-
 Z-AGLQR-7-amido-4-methylcoumarin pH 7.4, 37°C, recombinant mutant K628Q Homo sapiens