BRENDA - Enzyme Database
show all sequences of 3.4.21.41

Purified proenzyme C1r. Some characteristics of its activation and subsequent proteolytic cleavage

Arlaud, G.J.; Villiers, C.L.; Chesne, S.; Colomb, M.G.; Biochim. Biophys. Acta 616, 116-129 (1980)

Data extracted from this reference:

General Stability
General Stability
Organism
after reduction and alkylation C1rbar undergoes proteolytic cleavage which lead to the successive removal of two fragments of 35000 Da and of 7000-10000 Da, leaving a divalent molecule of reduced size. The product C1rbar II retains the original antigenic properties of C1rbar and a functional active site, but loses the capacity to bind C1s
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
proenzyme C1r
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
plasma
-
Homo sapiens
-
General Stability (protein specific)
General Stability
Organism
after reduction and alkylation C1rbar undergoes proteolytic cleavage which lead to the successive removal of two fragments of 35000 Da and of 7000-10000 Da, leaving a divalent molecule of reduced size. The product C1rbar II retains the original antigenic properties of C1rbar and a functional active site, but loses the capacity to bind C1s
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
proenzyme C1r
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
plasma
-
Homo sapiens
-
Other publictions for EC 3.4.21.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732102
Wijeyewickrema
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288
15571-15580
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Venkatraman Girija
Structural basis of the C1q/C1 ...
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Proc. Natl. Acad. Sci. USA
110
13916-13920
2013
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732824
Bally
Expression of recombinant huma ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
110
8650-8655
2013
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732889
Beveridge
A molecular dynamics study of ...
Homo sapiens
Proteins
80
1987-1997
2012
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717587
Lang
Intermodule cooperativity in t ...
Homo sapiens
FEBS J.
277
3986-3998
2010
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717603
Lang
Interaction between separated ...
Homo sapiens
FEBS Lett.
584
4565-4569
2010
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717786
Major
Calcium-dependent conformation ...
Homo sapiens
J. Biol. Chem.
285
11863-11869
2010
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717805
Brier
Mapping surface accessibility ...
Homo sapiens
J. Biol. Chem.
285
32251-32263
2010
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718116
Valck
Molecular mechanisms involved ...
Homo sapiens
Mol. Immunol.
47
1516-1521
2010
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718117
Kaplan
The plasma bradykinin-forming ...
Homo sapiens
Mol. Immunol.
47
2161-2169
2010
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683195
Duncan
The initiating proteases of th ...
Homo sapiens
Biochimie
90
387-395
2008
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683889
Kardos
Revisiting the mechanism of th ...
Homo sapiens
Mol. Immunol.
45
1752-1760
2008
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696932
Gaultier
Regulation of tumor necrosis f ...
Mus musculus
Blood
111
5316-5325
2008
1
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697529
Goesswein
Mutational spectrum of the C1I ...
Homo sapiens
Cytogenet. Genome Res.
121
181-188
2008
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698805
Geach
Molecular determinants of Xoll ...
Xenopus laevis
J. Biol. Chem.
283
27057-27063
2008
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700245
Davis
Biological activities of C1 in ...
Homo sapiens
Mol. Immunol.
45
4057-4063
2008
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1
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683530
Biro
Studies on the interactions be ...
Homo sapiens
Immunology
121
40-50
2007
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650989
Budayova-Spano
The crystal structure of the z ...
Homo sapiens
EMBO J.
21
231-239
2002
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653898
Budayova-Spano
Monomeric structures of the zy ...
Homo sapiens
Structure
10
1509-1519
2002
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652176
Lacroix
Assembly and enzymatic propert ...
Homo sapiens
J. Biol. Chem.
276
36233-36240
2001
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652680
Kardos
The role of the individual dom ...
Homo sapiens
J. Immunol.
167
5202-5208
2001
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81405
Dobo
One active C1r subunit is suff ...
Homo sapiens
J. Immunol.
162
1108-1112
1999
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81391
Gal
Structure and function of the ...
Homo sapiens
Immunobiology
199
317-326
1998
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81388
Thielens
Activation of human complement ...
Homo sapiens
Biochem. J.
301
509-516
1994
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81390
Illy
Chemical characterization and ...
Homo sapiens
J. Protein Chem.
12
771-781
1993
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81400
Katz
Regulation of synthesis of com ...
Homo sapiens
Clin. Immunol. Immunopathol.
67
117-123
1993
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81401
Zavodszky
Protein engineering studies on ...
Homo sapiens
Behring Inst. Mitt.
93
103-114
1993
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81402
Gulati
Regulation of the synthesis of ...
Homo sapiens
Behring Inst. Mitt.
93
196-203
1993
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1
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5
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5
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-
81403
Arlaud
Human complement serine protea ...
Homo sapiens
Methods Enzymol.
223
61-82
1993
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1
6
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1
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2
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1
1
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1
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4
1
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1
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1
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1
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6
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1
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1
1
-
1
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4
1
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1
-
1
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-
81404
Kam
Substituted isocoumarins as in ...
Homo sapiens
J. Immunol.
149
163-168
1992
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3
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1
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3
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-
81399
Thielens
Ca2+ binding properties and Ca ...
Homo sapiens
J. Biol. Chem.
265
14469-14475
1990
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1
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1
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1
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81386
Busby
Calcium-sensitive thermal tran ...
Homo sapiens
Biochemistry
26
5564-5571
1987
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2
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1
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1
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1
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81387
Journet
Cloning and sequencing of full ...
Homo sapiens
Biochem. J.
240
783-787
1986
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1
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1
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1
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1
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1
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81397
Reboul
Characteristics of complement ...
Homo sapiens
Biochem. J.
233
559-564
1986
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1
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1
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1
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81389
Loos
-
C1rbar-esterase ...
Homo sapiens
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
5
514-527
1984
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3
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1
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3
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3
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1
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1
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3
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81396
Nilsson
Kinetics of the reaction betwe ...
Homo sapiens
Eur. J. Biochem.
129
663-667
1983
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1
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1
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81395
Sim
The human complement system se ...
Homo sapiens
Methods Enzymol.
80
26-42
1981
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5
1
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1
1
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2
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2
1
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4
2
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1
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5
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1
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1
1
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2
1
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4
2
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1
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81394
Sim
Kinetics of reaction of human ...
Homo sapiens
Biochim. Biophys. Acta
612
433-449
1980
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-
81398
Arlaud
Purified proenzyme C1r. Some c ...
Homo sapiens
Biochim. Biophys. Acta
616
116-129
1980
-
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1
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1
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1
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1
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1
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1
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81392
Andrews
The enzymatic nature of human ...
Homo sapiens
J. Immunol.
123
1403-1408
1979
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1
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5
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1
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3
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2
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1
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5
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3
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2
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81393
Sim
The structure and enzymic acti ...
Homo sapiens
Biochem. J.
163
219-227
1977
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2
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1
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1
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1
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1
1
1
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2
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1
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1
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1
1
1
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