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Literature summary for 3.4.21.4 extracted from

  • Zhou, L.; Ruan, M.; Cai, Q.; Liu, G.; Sun, L.; Su, W.; Cao, M.
    Purification, characterization and cDNA cloning of a trypsin from the hepatopancreas of snakehead (Channa argus) (2012), Comp. Biochem. Physiol. B, 161, 247-254.
    View publication on PubMed
Search for more literature for 3.4.21.4

Inhibitors

Inhibitors Comment Organism Structure
benzamidine 5 mM, 17.9% residual activity Channa argus
E-64 0.02 mM, 19.5% residual activity Channa argus
Lima bean trypsin inhibitor 0.002 mM, 7.9% residual activity Channa argus
mung bean trypsin inhibitor 0.003 mM, 3.6% residual activity Channa argus
Pefabloc SC 1 mM, 4.5% residual activity Channa argus
phenylmethylsulfonyl fluoride 10 mM, 4.5% residual activity Channa argus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00049
-
 benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
0.00059
-
 benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
0.00064
-
 benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
0.00092
-
 benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
0.00102
-
 benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 114% of initial activity Channa argus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 x * 22000, SDS-PAGE Channa argus

Organism

Organism UniProt Comment Textmining
Channa argus G3LUK8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a signal peptide of 15 residues and an activation peptide of 9 amino acid residues with a mature protein of 223 residues Channa argus

Purification (Commentary)

Purification (Comment) Organism
-
 Channa argus

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
 Channa argus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
502
-
 pH 8.0, 37°C Channa argus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O 94.8% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin + H2O 75.2% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Glu-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O 14.8% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O 51.4% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Leu-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O 44.5% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Leu-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
-
 Channa argus benzyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O 27.1% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O 33.5% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
additional information no substrates: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, Lys-7-amido-4-methylcoumarin and Arg-7-amido-4-methylcoumarin Channa argus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 22000, SDS-PAGE Channa argus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Channa argus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable below Channa argus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
72
-
 benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
76
-
 benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
80
-
 benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
92
-
 benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
148
-
 benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Channa argus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5
-
 Channa argus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
87200
-
 benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
122200
-
 benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
143300
-
 benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
144700
-
 benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
153900
-
 benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus