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Literature summary for 3.4.21.4 extracted from
- Purification and characterization of trypsin from the pyloric ceca of orange-spotted grouper, Epinephelus coioides (2011), Fish Physiol. Biochem., 38, 837-848.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane | 16.9% inhibition at 0.1 mM | Epinephelus coioides |  |
| EDTA | 14.2% inhibition at 2 mM | Epinephelus coioides | |
| iodoacetic acid | 16.5% inhibition at 1 mM; 1 mM, 16.5% inhibition | Epinephelus coioides | |
| additional information | no inhibition by N-ethylmaleimide | Epinephelus coioides | |
| N-p-tosyl-L-lysine chloromethyl ketone | 5 mM, 83% inhibition | Epinephelus coioides | |
| N-tosyl-L-lysine chloromethyl ketone | 82.9% inhibition at 5 mM | Epinephelus coioides | |
| NaCl | 69% inhibition at 0.6 M | Epinephelus coioides | |
| pepstatin A | 26% inhibition at 0.01 mM | Epinephelus coioides | |
| Soybean trypsin inhibitor | 1 mg/ml, 80% inhibition; 80.3% inhibition at 1 mg/ml | Epinephelus coioides |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
x * 24000, SDS-PAGE | Epinephelus coioides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Epinephelus coioides | - |
- |
- |
| Epinephelus coioides | G9JKF3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Epinephelus coioides |
| native enzyme 161.85fold by ammonium sulfate, ionic exchange chromatography, ultrafiltratrion, and benzamidine affinity chromatography | Epinephelus coioides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cecum | pyloric | Epinephelus coioides | - |
| intestine | anterior, middle, and hind | Epinephelus coioides | - |
| pyloric cecum | - |
Epinephelus coioides | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 43.24 | - |
purified enzyme, pH 8.0, 37°C | Epinephelus coioides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Nalpha-benzoyl-L-arginine ethyl ester + H2O | - |
Epinephelus coioides | Nalpha-benzoyl-L-arginine + ethanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24000, SDS-PAGE | Epinephelus coioides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Epinephelus coioides |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | 40% of maximal activity at 20°C, 30% at 70°C | Epinephelus coioides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | purified enzyme, stable | Epinephelus coioides |
| 60 | - |
purified enzyme, 52.63% of maximal activity remaining | Epinephelus coioides |
| 70 | - |
purified enzyme, 22.39% of maximal activity remaining | Epinephelus coioides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | - |
Epinephelus coioides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
purified enzyme, 53.55% of maximal activity remaining | Epinephelus coioides |
| 6 | 8 | purified enzyme, stable | Epinephelus coioides |
| 11 | - |
purified enzyme, 66.25% of maximal activity remaining | Epinephelus coioides |
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