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Literature summary for 3.4.21.4 extracted from
- Purification and characteristics of trypsin from masu salmon (Oncorhynchus masou) cultured in fresh-water (2009), Fish Physiol. Biochem., 36, 637-645.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic tree | Oncorhynchus masou |
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ stabilizes the enzyme | Oncorhynchus masou |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by EDTA, E-64, and pepstatin A | Oncorhynchus masou | |
| Nalpha-p-tosyl-L-lysine chloromethyl ketone | complete inhibition | Oncorhynchus masou |  |
| PMSF | complete inhibition | Oncorhynchus masou | |
| Soybean trypsin inhibitor | complete inhibition | Oncorhynchus masou |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stabilizes the enzyme | Oncorhynchus masou | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
x * 24000, SDS-PAGE | Oncorhynchus masou |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oncorhynchus masou | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 24fold from pyloric ceca by gel filtration and diethylaminoethyl cellulose chromatography | Oncorhynchus masou |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pyloric cecum | - |
Oncorhynchus masou | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 156 | - |
purified enzyme, pH 8.5, 60°C | Oncorhynchus masou |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Nalpha-4-tosyl-L-arginine methyl ester + H2O | - |
Oncorhynchus masou | Nalpha-4-tosyl-L-arginine + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24000, SDS-PAGE | Oncorhynchus masou |
| More | thermal stability and flexibility of fish trypsin is affected by its overall decrease in hydrophobicity and increase in surface hydrophilicity as compared to mammalian counterparts | Oncorhynchus masou |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Oncorhynchus masou |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | activity range | Oncorhynchus masou |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified enzyme, stable below | Oncorhynchus masou |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Oncorhynchus masou |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | activity range | Oncorhynchus masou |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
purified enzyme, unstable below | Oncorhynchus masou |
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