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Literature summary for 3.4.21.4 extracted from

  • Stefansson, B.; Helgadottir, L.; Olafsdottir, S.; Gudmundsdottir, A.; Bjarnason, J.B.
    Characterization of cold-adapted Atlantic cod (Gadus morhua) trypsin I--kinetic parameters, autolysis and thermal stability (2010), Comp. Biochem. Physiol. B, 155, 186-194.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
trypsin I DNA and amino acid sequence determination and analysis Gadus morhua

General Stability

General Stability Organism
autolysis is a contributing factor in the stability of trypsin I Gadus morhua

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin
-
Gadus morhua
Nalpha-tosyl-L-Lys-chloromethylketone
-
Gadus morhua
PMSF
-
Gadus morhua

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
0.009
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 9.0, 25°C Gadus morhua
0.013
-
MeOSuc-Ala-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
0.015
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 7.1, 25°C Gadus morhua
0.018
-
MeOSuc-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
0.027
-
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
0.034
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 6.0, 25°C Gadus morhua
0.05
-
N-benzoyl-DL-arginine 4-nitroanilide pH 8.0, 25°C Gadus morhua

Organism

Organism UniProt Comment Textmining
Gadus morhua
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native trypsin I by benzamidine affinity and anion exchange chromatography Gadus morhua

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide + H2O
-
Gadus morhua MeOSuc-Ala-Ala-Ala-Arg + 4-nitroaniline
-
?
MeOSuc-Ala-Ala-Arg 4-nitroanilide + H2O
-
Gadus morhua MeOSuc-Ala-Ala-Arg + 4-nitroaniline
-
?
MeOSuc-Ala-Arg 4-nitroanilide + H2O
-
Gadus morhua MeOSuc-Ala-Arg + 4-nitroaniline
-
?
additional information identification of autolytic cleavage sites within the trypsin I molecule, overview Gadus morhua ?
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
-
Gadus morhua N-benzoyl-DL-arginine + 4-nitroaniline
-
?
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide + H2O
-
Gadus morhua N-benzyloxycarbonyl-Gly-Pro-Arg + 4-nitroaniline
-
?

Synonyms

Synonyms Comment Organism
Atlantic cod trypsin I
-
Gadus morhua
trypsin I
-
Gadus morhua

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
Atlantic cod trypsin I is a highly cold-adapted enzyme Gadus morhua
25
-
assay at Gadus morhua

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
Gadus morhua trypsin I is less stable towards thermal unfolding than its mesophilic bovine analogue Gadus morhua
15 37 purified enzyme, activity is lost relatively rapidly at 37 °C with a half-life of 17 h, at 25 °C the half-life is 5.4 days, while at 15 °C the enzyme is much more stable with a half-life of 32.5 days Gadus morhua

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.34
-
N-benzoyl-DL-arginine 4-nitroanilide pH 8.0, 25°C Gadus morhua
18.3
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 6.0, 25°C Gadus morhua
24.9
-
MeOSuc-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
30.5
-
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
33.6
-
MeOSuc-Ala-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
48.9
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 9.0, 25°C Gadus morhua
53.1
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
54.5
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 7.1, 25°C Gadus morhua

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Gadus morhua

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
26.8
-
N-benzoyl-DL-arginine 4-nitroanilide pH 8.0, 25°C Gadus morhua
538.2
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 6.0, 25°C Gadus morhua
1130
-
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
1383
-
MeOSuc-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
2585
-
MeOSuc-Ala-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
3759
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 7.1, 25°C Gadus morhua
5433
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 9.0, 25°C Gadus morhua
7590
-
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua