Cloned (Comment) | Organism |
---|---|
trypsin I DNA and amino acid sequence determination and analysis | Gadus morhua |
General Stability | Organism |
---|---|
autolysis is a contributing factor in the stability of trypsin I | Gadus morhua |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarin | - |
Gadus morhua | |
Nalpha-tosyl-L-Lys-chloromethylketone | - |
Gadus morhua | |
PMSF | - |
Gadus morhua |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | pH 8.0, 25°C | Gadus morhua | |
0.009 | - |
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | pH 9.0, 25°C | Gadus morhua | |
0.013 | - |
MeOSuc-Ala-Ala-Arg 4-nitroanilide | pH 8.0, 25°C | Gadus morhua | |
0.015 | - |
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | pH 7.1, 25°C | Gadus morhua | |
0.018 | - |
MeOSuc-Ala-Arg 4-nitroanilide | pH 8.0, 25°C | Gadus morhua | |
0.027 | - |
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide | pH 8.0, 25°C | Gadus morhua | |
0.034 | - |
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | pH 6.0, 25°C | Gadus morhua | |
0.05 | - |
N-benzoyl-DL-arginine 4-nitroanilide | pH 8.0, 25°C | Gadus morhua |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gadus morhua | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native trypsin I by benzamidine affinity and anion exchange chromatography | Gadus morhua |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide + H2O | - |
Gadus morhua | MeOSuc-Ala-Ala-Ala-Arg + 4-nitroaniline | - |
? | |
MeOSuc-Ala-Ala-Arg 4-nitroanilide + H2O | - |
Gadus morhua | MeOSuc-Ala-Ala-Arg + 4-nitroaniline | - |
? | |
MeOSuc-Ala-Arg 4-nitroanilide + H2O | - |
Gadus morhua | MeOSuc-Ala-Arg + 4-nitroaniline | - |
? | |
additional information | identification of autolytic cleavage sites within the trypsin I molecule, overview | Gadus morhua | ? | - |
? | |
N-benzoyl-DL-arginine 4-nitroanilide + H2O | - |
Gadus morhua | N-benzoyl-DL-arginine + 4-nitroaniline | - |
? | |
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide + H2O | - |
Gadus morhua | N-benzyloxycarbonyl-Gly-Pro-Arg + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Atlantic cod trypsin I | - |
Gadus morhua |
trypsin I | - |
Gadus morhua |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Atlantic cod trypsin I is a highly cold-adapted enzyme | Gadus morhua |
25 | - |
assay at | Gadus morhua |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Gadus morhua trypsin I is less stable towards thermal unfolding than its mesophilic bovine analogue | Gadus morhua |
15 | 37 | purified enzyme, activity is lost relatively rapidly at 37 °C with a half-life of 17 h, at 25 °C the half-life is 5.4 days, while at 15 °C the enzyme is much more stable with a half-life of 32.5 days | Gadus morhua |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.34 | - |
N-benzoyl-DL-arginine 4-nitroanilide | pH 8.0, 25°C | Gadus morhua | |
18.3 | - |
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | pH 6.0, 25°C | Gadus morhua | |
24.9 | - |
MeOSuc-Ala-Arg 4-nitroanilide | pH 8.0, 25°C | Gadus morhua | |
30.5 | - |
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide | pH 8.0, 25°C | Gadus morhua | |
33.6 | - |
MeOSuc-Ala-Ala-Arg 4-nitroanilide | pH 8.0, 25°C | Gadus morhua | |
48.9 | - |
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | pH 9.0, 25°C | Gadus morhua | |
53.1 | - |
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | pH 8.0, 25°C | Gadus morhua | |
54.5 | - |
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | pH 7.1, 25°C | Gadus morhua |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Gadus morhua |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.8 | - |
N-benzoyl-DL-arginine 4-nitroanilide | pH 8.0, 25°C | Gadus morhua | |
538.2 | - |
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | pH 6.0, 25°C | Gadus morhua | |
1130 | - |
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide | pH 8.0, 25°C | Gadus morhua | |
1383 | - |
MeOSuc-Ala-Arg 4-nitroanilide | pH 8.0, 25°C | Gadus morhua | |
2585 | - |
MeOSuc-Ala-Ala-Arg 4-nitroanilide | pH 8.0, 25°C | Gadus morhua | |
3759 | - |
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | pH 7.1, 25°C | Gadus morhua | |
5433 | - |
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | pH 9.0, 25°C | Gadus morhua | |
7590 | - |
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide | pH 8.0, 25°C | Gadus morhua |