Literature summary for 3.4.21.4 extracted from

Ohshima, Y.; Suzuki, Y.; Nakatani, A.; Nohara, D.
Refolding of fully reduced bovine pancreatic trypsin (2008), J. Biosci. Bioeng., 106, 345-349.

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
the denatured trypsin is diluted with 1 M diethanolamine or other solutions to a final concentration of 100 nM at pH 8.2, in the case of fully reduced trypsin, the refolding solution also contains 3 mM glutathione and 1 mM oxidized glutathione, the addition of 1.5 M urea resulted in even less recovered activity despite counteracting the formation of precipitates, refolding from fully reduced trypsin is achieved with a substantial approximate 86% recovery of protein yield	Bos taurus

Renatured (Comment)

Organism

the denatured trypsin is diluted with 1 M diethanolamine or other solutions to a final concentration of 100 nM at pH 8.2, in the case of fully reduced trypsin, the refolding solution also contains 3 mM glutathione and 1 mM oxidized glutathione, the addition of 1.5 M urea resulted in even less recovered activity despite counteracting the formation of precipitates, refolding from fully reduced trypsin is achieved with a substantial approximate 86% recovery of protein yield

Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-alpha-p-tosyl-L-arginine methyl ester + H2O	-	Bos taurus	N-alpha-p-tosyl-L-arginine + methanol	-	?

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Release 2023.1 (January 2023)