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Literature summary for 3.4.21.4 extracted from

  • Pilpel, N.; Nezer, I.; Applebaum, S.W.; Heifetz, Y.
    Mating-increases trypsin in female Drosophila hemolymph (2008), Insect Biochem. Mol. Biol., 38, 320-330.
    View publication on PubMed
Search for more literature for 3.4.21.4

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol addition of 2 mM dithiothreitol greatly enhances the tryptic activity Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
Bowman-Birk inhibitor double-headed native trypsin/chymotrypsin inhibitor in soybeans, which inhibits trypsin and chymotrypsin in a non-competitive manner, 78% reduced activity in the presence of 0.0005 mM Bowman-Birk inhibitor Drosophila melanogaster
Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride inhibits trypsin specifically and irreversibly, 40% reduced activity in the presence of 1 mM Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DrmSP8-36 + H2O Drosophila melanogaster trypsin is the major endopeptidase regulating N-terminal truncated sex peptide DrmSP8-36 in hemolymph, the trypsin cleavage site R7-K8 is essential for release of the C-terminal fragment SP8-36 from sperm tail, the protein is cleaved more rapidly and completely in mated female hemolymph ?
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 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
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-
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Source Tissue

Source Tissue Comment Organism Textmining
hemolymph
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 Drosophila melanogaster
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DrmSP8-36 + H2O trypsin is the major endopeptidase regulating N-terminal truncated sex peptide DrmSP8-36 in hemolymph, the trypsin cleavage site R7-K8 is essential for release of the C-terminal fragment SP8-36 from sperm tail, the protein is cleaved more rapidly and completely in mated female hemolymph Drosophila melanogaster ?
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 ?
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
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 Drosophila melanogaster N-alpha-benzoyl-DL-Arg + 4-nitroaniline
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 ?
N-alpha-benzoyl-L-Phe-L-Val-L-Arg-4-nitroanilide + H2O
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 Drosophila melanogaster N-alpha-benzoyl-L-Phe-L-Val-L-Arg + 4-nitroaniline
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 ?
N-alpha-benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide + H2O
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 Drosophila melanogaster N-alpha-benzoyl-L-Pro-L-Phe-L-Arg + 4-nitroaniline
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 ?