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Literature summary for 3.4.21.4 extracted from

  • Krenkova, J.; Lacher, N.A.; Svec, F.
    Highly efficient enzyme reactors containing trypsin and endoproteinase LysC immobilized on porous polymer monolith coupled to MS suitable for analysis of antibodies (2009), Anal. Chem., 81, 2004-2012.
    View publication on PubMed
Search for more literature for 3.4.21.4

General Stability

General Stability Organism
the immobilized trypsin reactor on porous polymer monoliths maintains its activity for at least 80 h with a slight decrease from 80 to 67% in sequence coverage for bovine serum albumin during this time period Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile partial inhibition at 20% acetonitrile, a 5% aqueous acetonitrile solution less likely reduces the activity of the porous polymer monolith-immobilized trypsin Bos taurus
benzamidine competitive inhibitor Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Bovine serum albumin + H2O
-
 Bos taurus ?
-
 ?
Cytochrome c + H2O
-
 Bos taurus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 4.5 min at 25°C is sufficient for the digestion with immobilized enzyme reactor on porous polymer monoliths Bos taurus
37
-
 digestion with trypsin in solution requires a temperature of 37°C and 24 h Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 10 the optimum pH for digestion with immobilized trypsin on porous polymer monoliths is in a pH range 8-10 and comparable with that found for its soluble counterpart Bos taurus