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Literature summary for 3.4.21.4 extracted from

  • Klomklao, S.; Benjakul, S.; Visessanguan, W.; Kishimura, H.; Simpson, B.K.; Saeki, H.
    Trypsins from yellowfin tuna (Thunnus albacores) spleen: purification and characterization (2006), Comp. Biochem. Physiol. B Biochem. Mol. Biol., 144, 47-56.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by 1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane, N-ethylmaleimide, iodoacetic acid, N-tosyl-L-phenyl-alanine-chloromethyl ketone, and pepstatin Thunnus albacares
N-tosyl-L-lysine-chloromethyl ketone complete inhibition Thunnus albacares
NaCl activity decreases with increasing NaCl concentrations (0-30%) Thunnus albacares
Soybean trypsin inhibitor effective inhibitor Thunnus albacares

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
N-alpha-p-tosyl-L-arginine methyl ester trypsin B, at pH 8.0 and 30°C Thunnus albacares
0.33
-
N-alpha-p-tosyl-L-arginine methyl ester trypsin A, at pH 8.0 and 30°C Thunnus albacares

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stabilized in the presence of 2 mM Ca2+ Thunnus albacares

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
SDS-PAGE and gel filtration Thunnus albacares

Organism

Organism UniProt Comment Textmining
Thunnus albacares
-
yellowfin tuna
-

Purification (Commentary)

Purification (Comment) Organism
Sephacryl S-200 gel filtration, Sephadex G-50 gel filtration and DEAE-cellulose column chromatography Thunnus albacares

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
Thunnus albacares
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
crude extract, using N-alpha-p-tosyl-L-arginine methyl ester as a substrate, at pH 8.0 and 30°C Thunnus albacares
7.77
-
trypsin A after 70.6fold purification, using N-alpha-p-tosyl-L-arginine methyl ester as a substrate, at pH 8.0 and 30°C Thunnus albacares
10.07
-
trypsin B after 91.5fold purification, using N-alpha-p-tosyl-L-arginine methyl ester as a substrate, at pH 8.0 and 30°C Thunnus albacares

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-p-tosyl-L-arginine methyl ester + H2O
-
Thunnus albacares N-alpha-p-tosyl-L-arginine + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 24000, SDS-PAGE and gel filtration Thunnus albacares

Synonyms

Synonyms Comment Organism
trypsin A
-
Thunnus albacares
trypsin B
-
Thunnus albacares

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
trypsin A Thunnus albacares
65
-
trypsin B Thunnus albacares

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
trypsin A and B are heat stable up to 50°C Thunnus albacares

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
66.7
-
N-alpha-p-tosyl-L-arginine methyl ester trypsin B, at pH 8.0 and 30°C Thunnus albacares
80
-
N-alpha-p-tosyl-L-arginine methyl ester trypsin A, at pH 8.0 and 30°C Thunnus albacares

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Thunnus albacares

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
Thunnus albacares

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
trypsin B shows no activity, while trypsin A retains about 50% of activity at pH 4 Thunnus albacares
6 11
-
Thunnus albacares