Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.4 extracted from

  • Wu, Z.; Jiang, G.; Xiang, P.; Yang, D.; Wang, N.
    Purification and characterization of trypsin-like enzymes from North Pacific krill (Euphausia pacifica) (2008), Biotechnol. Lett., 30, 67-72.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
N-alpha-benzoyl-L-arginine-p-nitroanilide isozyme TLE II, at 37°C, pH 8.0 Euphausia pacifica
0.09
-
N-alpha-benzoyl-L-arginine-p-nitroanilide isozyme TLE I, at 37°C, pH 8.0 Euphausia pacifica

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates at concentrations above 0.4 mM Euphausia pacifica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32300
-
TLE II, SDS-PAGE Euphausia pacifica
33000
-
TLE I, SDS-PAGE Euphausia pacifica

Organism

Organism UniProt Comment Textmining
Euphausia pacifica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sepharose FF column chromatography and Sephacryl S100 gel filtration Euphausia pacifica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.52
-
crude extract, at 37°C, pH 8.0 Euphausia pacifica
64
-
isozyme TLE I after 123fold purification, at 37°C, pH 8.0 Euphausia pacifica
92
-
isozyme TLE II after 177fold purification, at 37°C, pH 8.0 Euphausia pacifica

Storage Stability

Storage Stability Organism
25°C, crude extract, 30 days, retains more than 70% of the initial activity Euphausia pacifica
40°C, crude extract, 5-10 days, retains about 80% of the initial activity Euphausia pacifica
40°C, purified enzyme, 30 min, remains stable Euphausia pacifica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O
-
Euphausia pacifica N-alpha-benzoyl-L-arginine + p-nitroaniline
-
?

Synonyms

Synonyms Comment Organism
TLE I
-
Euphausia pacifica
TLE II
-
Euphausia pacifica
trypsin-like enzyme
-
Euphausia pacifica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 50
-
Euphausia pacifica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 70
-
Euphausia pacifica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
activity is lost rapidly over 50°C Euphausia pacifica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.84
-
N-alpha-benzoyl-L-arginine-p-nitroanilide isozyme TLE I, at 37°C, pH 8.0 Euphausia pacifica
0.98
-
N-alpha-benzoyl-L-arginine-p-nitroanilide isozyme TLE II, at 37°C, pH 8.0 Euphausia pacifica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10
-
Euphausia pacifica

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
Euphausia pacifica

pI Value

Organism Comment pI Value Maximum pI Value
Euphausia pacifica TLE II, isoelectric focusing
-
4.3
Euphausia pacifica TLE I, isoelectric focusing
-
4.5