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Literature summary for 3.4.21.4 extracted from

  • Toyota, E.; Iyaguchi, D.; Sekizaki, H.; Itoh, K.; Tanizawa, K.
    Kinetic properties of three isoforms of trypsin isolated from the pyloric caeca of chum salmon (Oncorhynchus keta) (2007), Biol. Pharm. Bull., 30, 1648-1652.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
Tos-L-Arg-OMe at 25°C Bos taurus
0.01
-
Tos-L-Arg-OMe at 5°C Bos taurus
0.01
-
Tos-L-Arg-OMe at 15°C Bos taurus
0.02
-
Tos-L-Arg-OMe at 35°C Bos taurus
0.02
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 5°C Oncorhynchus keta
0.02
-
Tos-L-Arg-OMe isozyme ST-1, at 5°C Oncorhynchus keta
0.02
-
Tos-L-Arg-OMe isozyme ST-2, at 15°C Oncorhynchus keta
0.02
-
Tos-L-Arg-OMe isozyme ST-2, at 25°C Oncorhynchus keta
0.02
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 5°C Oncorhynchus keta
0.02
-
Tos-L-Arg-OMe isozyme ST-2, at 5°C Oncorhynchus keta
0.02
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 15°C Oncorhynchus keta
0.02
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 25°C Oncorhynchus keta
0.02
-
Tos-L-Arg-OMe isozyme ST-3, at 25°C Oncorhynchus keta
0.02
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 5°C Oncorhynchus keta
0.02
-
Tos-L-Arg-OMe isozyme ST-3, at 5°C Oncorhynchus keta
0.03
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 15°C Oncorhynchus keta
0.03
-
Tos-L-Arg-OMe isozyme ST-1, at 15°C Oncorhynchus keta
0.03
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 25°C Oncorhynchus keta
0.03
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 15°C Oncorhynchus keta
0.03
-
Tos-L-Arg-OMe isozyme ST-2, at 35°C Oncorhynchus keta
0.03
-
Tos-L-Arg-OMe isozyme ST-3, at 15°C Oncorhynchus keta
0.04
-
Tos-L-Arg-OMe isozyme ST-1, at 25°C Oncorhynchus keta
0.04
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 25°C Oncorhynchus keta
0.04
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 35°C Oncorhynchus keta
0.04
-
Tos-L-Arg-OMe isozyme ST-3, at 35°C Oncorhynchus keta
0.05
-
Tos-L-Arg-OMe isozyme ST-1, at 35°C Oncorhynchus keta
0.05
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 35°C Oncorhynchus keta
0.07
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 35°C Oncorhynchus keta
0.2
-
benzoyl-L-arginine-p-nitroanilide at 5°C Bos taurus
0.26
-
benzoyl-L-arginine-p-nitroanilide at 25°C Bos taurus
0.27
-
benzoyl-L-arginine-p-nitroanilide at 15°C Bos taurus
0.44
-
benzoyl-L-arginine-p-nitroanilide at 35°C Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
SDS-PAGE Oncorhynchus keta

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Oncorhynchus keta
-
chum salmon
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, acetone precipitation, benzamidine-Sepharose 6B affinity column chromatography, Sephadex G-75 gel filtration, and DEAE-Sepharose column chromatography Oncorhynchus keta

Source Tissue

Source Tissue Comment Organism Textmining
pyloric cecum
-
Oncorhynchus keta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.23
-
clarified extract, at 25°C Oncorhynchus keta
5.56
-
isozyme ST-3, after 24.2fold purification, at 25°C Oncorhynchus keta
6.23
-
isozyme ST-2, after 27.1fold purification, at 25°C Oncorhynchus keta
6.43
-
isozyme ST-1, after 28fold purification, at 25°C Oncorhynchus keta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-L-arginine-p-nitroanilide + H2O
-
Bos taurus benzoyl-L-arginine + p-nitroaniline
-
?
benzoyl-L-arginine-p-nitroanilide + H2O
-
Oncorhynchus keta benzoyl-L-arginine + p-nitroaniline
-
?
Tos-L-Arg-OMe + H2O
-
Bos taurus ?
-
?
Tos-L-Arg-OMe + H2O
-
Oncorhynchus keta ?
-
?

Synonyms

Synonyms Comment Organism
ST-1 isozyme Oncorhynchus keta
ST-2 isozyme Oncorhynchus keta
ST-3 isozyme Oncorhynchus keta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
benzoyl-L-arginine-p-nitroanilide at 5°C Bos taurus
0.64
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 5°C Oncorhynchus keta
0.76
-
benzoyl-L-arginine-p-nitroanilide at 15°C Bos taurus
0.83
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 5°C Oncorhynchus keta
0.94
-
benzoyl-L-arginine-p-nitroanilide at 25°C Bos taurus
1.06
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 5°C Oncorhynchus keta
1.27
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 15°C Oncorhynchus keta
2
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 25°C Oncorhynchus keta
2.43
-
benzoyl-L-arginine-p-nitroanilide at 35°C Bos taurus
2.72
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 15°C Oncorhynchus keta
2.74
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 15°C Oncorhynchus keta
3 6 benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 15°C Oncorhynchus keta
3.13
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 25°C Oncorhynchus keta
3.86
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 35°C Oncorhynchus keta
4.06
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 25°C Oncorhynchus keta
5.54
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 25°C Oncorhynchus keta
6.74
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 35°C Oncorhynchus keta
7.7
-
benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 35°C Oncorhynchus keta
41.3
-
Tos-L-Arg-OMe isozyme ST-1, at 5°C Oncorhynchus keta
46.2
-
Tos-L-Arg-OMe at 5°C Bos taurus
61.7
-
Tos-L-Arg-OMe isozyme ST-2, at 5°C Oncorhynchus keta
64.5
-
Tos-L-Arg-OMe isozyme ST-1, at 15°C Oncorhynchus keta
68.4
-
Tos-L-Arg-OMe isozyme ST-3, at 5°C Oncorhynchus keta
69.2
-
Tos-L-Arg-OMe at 15°C Bos taurus
94.3
-
Tos-L-Arg-OMe isozyme ST-1, at 25°C Oncorhynchus keta
94.3
-
Tos-L-Arg-OMe isozyme ST-1, at 35°C Oncorhynchus keta
105
-
Tos-L-Arg-OMe isozyme ST-2, at 15°C Oncorhynchus keta
109
-
Tos-L-Arg-OMe at 25°C Bos taurus
139
-
Tos-L-Arg-OMe at 35°C Bos taurus
154
-
Tos-L-Arg-OMe isozyme ST-3, at 15°C Oncorhynchus keta
159
-
Tos-L-Arg-OMe isozyme ST-3, at 25°C Oncorhynchus keta
189
-
Tos-L-Arg-OMe isozyme ST-3, at 35°C Oncorhynchus keta
200
-
Tos-L-Arg-OMe isozyme ST-2, at 35°C Oncorhynchus keta
234
-
Tos-L-Arg-OMe isozyme ST-2, at 25°C Oncorhynchus keta

pI Value

Organism Comment pI Value Maximum pI Value
Oncorhynchus keta isozyme ST-2, isoelectric focusing
-
5.4
Oncorhynchus keta isozyme ST-3, isoelectric focusing
-
5.6
Oncorhynchus keta isozyme ST-1, isoelectric focusing
-
5.8