General Stability | Organism |
---|---|
modification by biacetyl does not improve thermostability of SGT | Streptomyces griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23110 | - |
MALDI-TOF mass spectrometry | Streptomyces griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | P00775 | precursor | - |
Purification (Comment) | Organism |
---|---|
SP-Sepharose Fast Flow column chromatography and Arg-Sepharose 4B Fast Flow column chromatography | Streptomyces griseus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.34 | - |
after 1fold purification, using N-benzoyl-DL-arginine-4-nitroanilide as a substrate | Streptomyces griseus |
9.42 | - |
after 27.6fold purification, using N-benzoyl-DL-arginine-4-nitroanilide as a substrate | Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-amylase + H2O | - |
Streptomyces griseus | ? | - |
? | |
Bovine serum albumin + H2O | - |
Streptomyces griseus | ? | - |
? | |
carbonic anhydrase + H2O | - |
Streptomyces griseus | ? | - |
? | |
Cytochrome c + H2O | - |
Streptomyces griseus | ? | - |
? | |
glucose oxidase + H2O | - |
Streptomyces griseus | ? | - |
? | |
glycogen phosphorylase b + H2O | - |
Streptomyces griseus | ? | - |
? | |
Hemoglobin + H2O | - |
Streptomyces griseus | ? | - |
? | |
Lysozyme + H2O | - |
Streptomyces griseus | ? | - |
? | |
Myoglobin + H2O | - |
Streptomyces griseus | ? | - |
? | |
N-benzoyl-DL-arginine-4-nitroanilide + H2O | - |
Streptomyces griseus | N-benzoyl-DL-arginine + 4-nitroaniline | - |
? | |
ovalbumin + H2O | - |
Streptomyces griseus | ? | - |
? | |
peroxidase + H2O | - |
Streptomyces griseus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SGT | - |
Streptomyces griseus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
50% of the activity is retained on 30 min incubation at 42°C | Streptomyces griseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
N-benzoyl-DL-arginine-4-nitroanilide | - |
Streptomyces griseus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces griseus | isoelectric focusing | - |
6.8 |