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Literature summary for 3.4.21.4 extracted from

  • Leiros, H.K.S.; Brandsdal, B.O.; Andersen, O.A.; Os, V.; Leiros, I.; Helland, R.; Otlewski, J.; Willassen, N.P.; Smalas, A.O.
    Trypsin specificity as elucidated by LIE calculations, X-ray structures, and association constant measurements (2004), Protein Sci., 13, 1056-1070.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.4

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the enzyme-benzylamine complex, enzyme-phenylethylamine complex and the enzyme-phenylbutylamine complex Bos taurus
crystal structure of the enzyme-benzylamine complex, enzyme-phenylethylamine complex and the enzyme-phenylpropylamine complex salmon

Inhibitors

Inhibitors Comment Organism Structure
benzylamine
-
 Bos taurus
benzylamine
-
 salmon
phenylbutylamine
-
 Bos taurus
phenylbutylamine
-
 salmon
Phenylethylamine
-
 Bos taurus
Phenylethylamine
-
 salmon
Phenylpropylamine
-
 Bos taurus
Phenylpropylamine
-
 salmon

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00017
-
 tosyl-Gly-Pro-Arg-4-nitroanilide pH 8.3 Bos taurus
0.000896
-
 tosyl-Gly-Pro-Arg-4-nitroanilide pH 8.3 salmon

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
salmon
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
-
 Bos taurus ?
-
 ?
tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
-
 salmon ?
-
 ?

Synonyms

Synonyms Comment Organism
anionic salmon trypsin
-
 salmon
AST
-
 salmon
BT
-
 Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
28
-
 tosyl-Gly-Pro-Arg-4-nitroanilide pH 8.3 salmon
30.4
-
 tosyl-Gly-Pro-Arg-4-nitroanilide pH 8.3 salmon
88
-
 tosyl-Gly-Pro-Arg-4-nitroanilide pH 8.3 Bos taurus