Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.4 extracted from

  • Pal, G.; Patthy, A.; Antal, J.; Graf, L.
    Mutant rat trypsin selectively cleaves tyrosyl peptide bonds (2004), Anal. Biochem., 326, 190-199.
    View publication on PubMed
Search for more literature for 3.4.21.4

Application

Application Comment Organism
analysis the double mutant trypsinogen D189S/DELTA223 lacks trypsin-like activity but aquires a rather unique selectivity, it preferentially hydrolyses peptide bonds C-terminal to tyrosyl residues. This narrow specificity should be useful in peptide-analytical applications such as sequence-specific fragmentation of large proteins prior to sequencing Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D189S/DELTA223 double mutant trypsinogen, the recombinant proteinase lacks trypsin-like activity but aquires a rather unique selectivity, it preferentially hydrolyses peptide bonds C-terminal to tyrosyl residues. This narrow specificity should be useful in peptide-analytical applications such as sequence-specific fragmentation of large proteins prior to sequencing Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000167
-
 succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, mutant enzyme D189S/DELTA223 Rattus norvegicus
0.00167
-
 succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, mutant enzyme D189S/DELTA223 Rattus norvegicus
0.00217
-
 succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, mutant enzyme D189S/DELTA223 Rattus norvegicus
0.004
-
 succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin pH 8.0, 37°C, mutant enzyme D189S/DELTA223 Rattus norvegicus
0.004
-
 succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin pH 8.0, 37°C, mutant enzyme D189S/DELTA223 Rattus norvegicus
0.07
-
 succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, wild-type enzyme Rattus norvegicus
0.08
-
 succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin pH 8.0, 37°C, mutant enzyme D189S/DELTA223 Rattus norvegicus
0.1
-
 succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin pH 8.0, 37°C, wild-type enzyme Rattus norvegicus
0.2
-
 succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin pH 8.0, 37°C, wild-type enzyme Rattus norvegicus
0.31
-
 succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, wild-type enzyme Rattus norvegicus
0.78
-
 succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin pH 8.0, 37°C, wild-type enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
double-mutant trypsinogen D189S/DELTA223 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus succinyl-Ala-Ala-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus succinyl-Ala-Ala-Pro-Leu + 7-amino-4-methylcoumarin
-
 ?
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus succinyl-Ala-Ala-Pro-Lys + 7-amino-4-methylcoumarin
-
 ?
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
-
 ?
succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus succinyl-Ala-Ala-Pro-Trp + 7-amino-4-methylcoumarin
-
 ?
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus succinyl-Ala-Ala-Pro-Tyr + 7-amino-4-methylcoumarin
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000167
-
 succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, wild-type enzyme Rattus norvegicus
0.000167
-
 succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, mutant enzyme D189S/DELTA223 Rattus norvegicus
0.00067
-
 succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin pH 8.0, 37°C, wild-type enzyme Rattus norvegicus
0.00167
-
 succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, mutant enzyme D189S/DELTA223 Rattus norvegicus
0.00217
-
 succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, mutant enzyme D189S/DELTA223 Rattus norvegicus
0.004
-
 succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin pH 8.0, 37°C, mutant enzyme D189S/DELTA223 Rattus norvegicus
0.004
-
 succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin pH 8.0, 37°C, mutant enzyme D189S/DELTA223 Rattus norvegicus
0.0063
-
 succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin pH 8.0, 37°C, wild-type enzyme Rattus norvegicus
0.08
-
 succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin pH 8.0, 37°C, mutant enzyme D189S/DELTA223 Rattus norvegicus
73
-
 succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin pH 8.0, 37°C, wild-type enzyme Rattus norvegicus
91
-
 succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, wild-type enzyme Rattus norvegicus
121.2
-
 succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin pH 8.0, 37°C, wild-type enzyme Rattus norvegicus