Literature summary for 3.4.21.39 extracted from

Fu, Z.; Akula, S.; Thorpe, M.; Chahal, G.; de Garavilla, L.; Kervinen, J.; Hellman, L.
Extended cleavage specificity of sheep mast cell protease-2 A classical chymase with preference to aromatic P1 substrate residues (2019), Dev. Comp. Immunol., 92, 160-169 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
enterokinase	-	Ovis aries

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in baculovirus-infected insect cells	Ovis aries

Organism

Organism	UniProt	Comment	Textmining
Ovis aries	P79204	-	-

Purification (Commentary)

Purification (Comment)	Organism
heparin-Sepharose column chromatography and phenyl HP column chromatography	Ovis aries

Source Tissue

Source Tissue	Comment	Organism	Textmining
mast cell	-	Ovis aries	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	no activity with succinyl-AAPI-4-nitroanilide, succinyl-AAPA-4-nitroanilide, succinyl-AAPV-4-nitroanilide, succinyl-VLGR-4-nitroanilide, benzyloxycarbonyl-GPR-4-nitroanilide, acetyl-YVAD-4-nitroanilide, acetyl-VEID-4-nitroanilide, and acetyl-IEPD-4-nitroanilide	Ovis aries	?	-	?
additional information	the enzyme shows preference for aromatic residues (Phe, Tyr, Trp) in the P1 position of substrates and a preference for aliphatic residues in most other substrate positions around the cleavage site. The enzyme also cleaves, albeit with relatively low efficiency, after Leu in the P1 position and exhibits unspecific preference in the P2' position	Ovis aries	?	-	?
succinyl-AAPF-4-nitroanilide + H2O	-	Ovis aries	succinyl-AAPF + 4-nitroaniline	-	?
succinyl-AAPL-4-nitroanilide + H2O	low activity	Ovis aries	succinyl-AAPL + 4-nitroaniline	-	?
succinyl-LLVY-4-nitroanilide + H2O	-	Ovis aries	succinyl-LLVY + 4-nitroaniline	-	?

Subunits

Subunits	Comment	Organism
?	x * 26000, SDS-PAGE	Ovis aries

Synonyms

Synonyms	Comment	Organism
mast cell protease-2	-	Ovis aries
MCP2	-	Ovis aries

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Release 2023.1 (January 2023)