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Literature summary for 3.4.21.39 extracted from

  • Smith, E.T.; Perry, E.T.; Sears, M.B.; Johnson, D.A.
    Expression of recombinant human mast cell chymase with Asn-linked glycans in glycoengineered Pichia pastoris (2014), Protein Expr. Purif., 102, 69-75.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.39

Cloned(Commentary)

Cloned (Comment) Organism
expressed in the SuperMan5 strain of GlycoSwitch Pichia pastoris Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Eglin c potent inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0157
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester in the presence of heparin, at pH 7.4 and 37°C Homo sapiens
0.0355
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester in the absence of heparin, at pH 7.4 and 37°C Homo sapiens
0.514
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide in the presence of heparin, at pH 7.4 and 37°C Homo sapiens
0.935
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide in the absence of heparin, at pH 7.4 and 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, deglycosylated recombinant enzyme, SDS-PAGE Homo sapiens
30000
-
 x * 30000, recombinant enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
angiotensin I + H2O Homo sapiens
-
 angiotensin II + His-Leu
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
heparin Hyper M column chromatography and heparin affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O
-
 Homo sapiens angiotensin II + His-Leu
-
 ?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Homo sapiens succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline
-
 ?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, recombinant enzyme, SDS-PAGE Homo sapiens
? x * 25000, deglycosylated recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
mast cell chymase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide in the presence of heparin, at pH 7.4 and 37°C Homo sapiens
3.6
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide in the absence of heparin, at pH 7.4 and 37°C Homo sapiens
12.6
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester in the absence of heparin, at pH 7.4 and 37°C Homo sapiens
17.8
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester in the presence of heparin, at pH 7.4 and 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.85
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide in the absence of heparin, at pH 7.4 and 37°C Homo sapiens
4.86
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide in the presence of heparin, at pH 7.4 and 37°C Homo sapiens
355
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester in the absence of heparin, at pH 7.4 and 37°C Homo sapiens
1130
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester in the presence of heparin, at pH 7.4 and 37°C Homo sapiens