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Literature summary for 3.4.21.39 extracted from

  • Ahooghalandari, P.; Hanke, N.; Thorpe, M.; Witte, A.; Messinger, J.; Hellman, L.
    Mutations in Arg143 and Lys192 of the human mast cell chymase markedly affect the activity of five potent human chymase inhibitors (2013), PLoS ONE, 8, e65988.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes asre expressed in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K192M the mutant exhibits a markedly reduced sensitivity towards inhibitors compared to the wild type enzyme Homo sapiens
R143Q the mutant exhibits a markedly reduced sensitivity towards inhibitors compared to the wild type enzyme Homo sapiens
R143Q/K192M the mutant exhibits a markedly reduced sensitivity towards inhibitors compared to the wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-[(5-fluoro-1-benzothiophen-3-yl)methyl]-3-{[(methoxycarbonyl)(methyl)amino]methyl}-1H-indole-2-carboxylate
-
Homo sapiens
2-{1-[(1,4-dimethyl-1H-indol-3-yl)methyl]-2,4-dioxo-1,4-dihydroquinazolin-3(2H)-yl}hexanoic acid
-
Homo sapiens
4-({1-[(4-methyl-1-benzothiophen-3-yl)methyl]-1H-benzimidazol-2-yl}sulfanyl)butanoic acid
-
Homo sapiens
TY51184
-
Homo sapiens
[1-(5-chloro-1-methyl-1H-indol-3-yl)-2-(naphthalen-2-ylamino)-2-oxoethyl]phosphonate
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
x * 26000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
angiotensin I + H2O Homo sapiens
-
angiotensin II + His-Leu
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P23946
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose beads chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O
-
Homo sapiens angiotensin II + His-Leu
-
?
succinyl-L-Leu-L-Leu-L-Val-L-Tyr-4-nitroanilide + H2O
-
Homo sapiens succinyl-L-Leu-L-Leu-L-Val-L-Tyr + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
mast cell chymase
-
Homo sapiens