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Literature summary for 3.4.21.39 extracted from

  • Gallwitz, M.; Enoksson, M.; Thorpe, M.; Ge, X.; Hellman, L.
    The extended substrate recognition profile of the dog mast cell chymase reveals similarities and differences to the human chymase (2010), Int. Immunol., 22, 421-431.
    View publication on PubMed
Search for more literature for 3.4.21.39

Cloned(Commentary)

Cloned (Comment) Organism
enterokinase cleavage site and His6-tag, expressed in HEK 293-EBNA cell Canis lupus familiaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, SDS-PAGE (active enzyme) Canis lupus familiaris

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Canis lupus familiaris

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+), activation by His6-tag removal by cleavage with enterokinase, heparin-Sepharose chromatography Canis lupus familiaris

Source Tissue

Source Tissue Comment Organism Textmining
mast cell
-
 Canis lupus familiaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methoxy-succinyl-Arg-Ala-Tyr-4-nitroanilide + H2O cleavage specificity as determined by substrate phage display P4: (Gly)Val, P3: Gly/Val, P2: Arg > Gly, P1: Phe > Tyr > Trp, P1': Leu > Val, P2': Ser > Glu/Asp > Leu, P3': Leu/Val Canis lupus familiaris methoxy-succinyl-Arg-Ala-Tyr + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Canis lupus familiaris N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE (active enzyme) Canis lupus familiaris

Synonyms

Synonyms Comment Organism
chymase
-
 Canis lupus familiaris