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Literature summary for 3.4.21.38 extracted from

  • Ivanov, I.; Matafonov, A.; Sun, M.F.; Cheng, Q.; Dickeson, S.K.; Verhamme, I.M.; Emsley, J.; Gailani, D.
    Proteolytic properties of single-chain factor XII a mechanism for triggering contact activation (2017), Blood, 129, 1527-1537 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
alpha-kallikrein
-
Homo sapiens
additional information the enzyme gets activated by contact when exposed to artificial surfaces and biological substances Homo sapiens
Polyphosphate
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
R334A/R343A/R353A the mutant lacks the Arg334, Arg343, and Arg353 cleavage sites required for conversion to the enzyme form betaFXIIa and shows proteolytic activity that is about 3000fold weaker than that of the wild type enzyme Homo sapiens
R353A the mutant lacks the Arg353 cleavage site required for conversion to the activated enzyme form alphaFXIIa and shows proteolytic activity that is several orders of magnitude weaker than that of the wild type enzyme Homo sapiens
S544A active site mutant Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
prekallikrein mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C Homo sapiens
0.0017
-
prekallikrein wild type enzyme alphaFXIIa, at pH 7.4 and 37°C Homo sapiens
0.1
-
S-2302 wild type enzyme alphaFXIIa, at pH 7.4 and 37°C Homo sapiens
2.7
-
S-2302 mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
factor XI + H2O Homo sapiens
-
factor XIa + ?
-
?
prekallikrein + H2O Homo sapiens
-
kallikrein + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor XI + H2O
-
Homo sapiens factor XIa + ?
-
?
prekallikrein + H2O
-
Homo sapiens kallikrein + ?
-
?
S-2302 + H2O
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
alphaFXIIa
-
Homo sapiens
single-chain FXII
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000062
-
prekallikrein mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C Homo sapiens
0.108
-
S-2302 mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C Homo sapiens
0.73
-
prekallikrein wild type enzyme alphaFXIIa, at pH 7.4 and 37°C Homo sapiens
11.7
-
S-2302 wild type enzyme alphaFXIIa, at pH 7.4 and 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.077
-
prekallikrein mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C Homo sapiens
0.4
-
S-2302 mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C Homo sapiens
116.7
-
S-2302 wild type enzyme alphaFXIIa, at pH 7.4 and 37°C Homo sapiens
4333
-
prekallikrein wild type enzyme alphaFXIIa, at pH 7.4 and 37°C Homo sapiens