Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-kallikrein | - |
Homo sapiens | |
additional information | the enzyme gets activated by contact when exposed to artificial surfaces and biological substances | Homo sapiens | |
Polyphosphate | - |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R334A/R343A/R353A | the mutant lacks the Arg334, Arg343, and Arg353 cleavage sites required for conversion to the enzyme form betaFXIIa and shows proteolytic activity that is about 3000fold weaker than that of the wild type enzyme | Homo sapiens |
R353A | the mutant lacks the Arg353 cleavage site required for conversion to the activated enzyme form alphaFXIIa and shows proteolytic activity that is several orders of magnitude weaker than that of the wild type enzyme | Homo sapiens |
S544A | active site mutant | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
prekallikrein | mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C | Homo sapiens | |
0.0017 | - |
prekallikrein | wild type enzyme alphaFXIIa, at pH 7.4 and 37°C | Homo sapiens | |
0.1 | - |
S-2302 | wild type enzyme alphaFXIIa, at pH 7.4 and 37°C | Homo sapiens | |
2.7 | - |
S-2302 | mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor XI + H2O | Homo sapiens | - |
factor XIa + ? | - |
? | |
prekallikrein + H2O | Homo sapiens | - |
kallikrein + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor XI + H2O | - |
Homo sapiens | factor XIa + ? | - |
? | |
prekallikrein + H2O | - |
Homo sapiens | kallikrein + ? | - |
? | |
S-2302 + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
alphaFXIIa | - |
Homo sapiens |
single-chain FXII | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000062 | - |
prekallikrein | mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C | Homo sapiens | |
0.108 | - |
S-2302 | mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C | Homo sapiens | |
0.73 | - |
prekallikrein | wild type enzyme alphaFXIIa, at pH 7.4 and 37°C | Homo sapiens | |
11.7 | - |
S-2302 | wild type enzyme alphaFXIIa, at pH 7.4 and 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.077 | - |
prekallikrein | mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C | Homo sapiens | |
0.4 | - |
S-2302 | mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C | Homo sapiens | |
116.7 | - |
S-2302 | wild type enzyme alphaFXIIa, at pH 7.4 and 37°C | Homo sapiens | |
4333 | - |
prekallikrein | wild type enzyme alphaFXIIa, at pH 7.4 and 37°C | Homo sapiens |