Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
Sprague-Dawley rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aorta | aortic endothelial cells | Bos taurus | - |
aorta | aortic smooth muscle cells | Rattus norvegicus | - |
artery | pulmonary artery endothelial cells | Bos taurus | - |
endothelial cell | pulmonary artery endothelial cells and aortic endothelial cells | Bos taurus | - |
lung | pulmonary artery endothelial cells | Bos taurus | - |
lung fibroblast | - |
Rattus norvegicus | - |
macrophage | - |
Mus musculus | - |
monocyte | - |
Mus musculus | - |
RAW-264.7 cell | - |
Mus musculus | - |
smooth muscle cell | neonatal, aortic | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | neutrophil elastase cleaves vascular endothelial growth factor, VEGF, to generate a VEGF fragment with altered activity. Recombinant human VEGF165 is a substrate for the enzyme. The neutrophil elastase-generated VEGF fragment shows significantly reduced binding to VEGF receptor 2 and heparin, yet retains the ability to bind to VEGF receptor 1. Substrate and product analysis by mass spectrometry | Rattus norvegicus | ? | - |
? | |
additional information | neutrophil elastase cleaves vascular endothelial growth factor, VEGF, to generate a VEGF fragment with altered activity. Recombinant human VEGF165 is a substrate for the enzyme. The neutrophil elastase-generated VEGF fragment shows significantly reduced binding to VEGF receptor 2 and heparin, yet retains the ability to bind to VEGF receptor 1. The VEGF fragment shows altered signaling in pulmonary artery endothelial cells compared with intact VEGF165, detailed overview. Substrate and product analysis by mass spectrometry. PDGF is also a substrate of neutrophil elastase, while TNFalpha and FGF2 are not | Bos taurus | ? | - |
? | |
additional information | neutrophil elastase cleaves vascular endothelial growth factor, VEGF, to generate a VEGF fragment with altered activity. Recombinant human VEGF165 is a substrate for the enzyme. The neutrophil elastase-generated VEGF fragment, VEGFf, and the wild-type VEGF both activate Akt, and VEGFf appears to be more effective. Substrate and product analysis by mass spectrometry | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
neutrophil elastase | - |
Mus musculus |
neutrophil elastase | - |
Rattus norvegicus |
neutrophil elastase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Bos taurus |
37 | - |
cell-based assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
7.4 | - |
assay at | Bos taurus |
7.4 | - |
cell-based assay at | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
malfunction | excessive neutrophil elastase activity and altered vascular endothelial growth factor signaling are independently in the development and progression of pulmonary emphysema | Mus musculus |
malfunction | excessive neutrophil elastase activity and altered vascular endothelial growth factor signaling are independently in the development and progression of pulmonary emphysema | Rattus norvegicus |
malfunction | excessive neutrophil elastase activity and altered vascular endothelial growth factor signaling are independently in the development and progression of pulmonary emphysema | Bos taurus |